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Literature summary extracted from
- Production and characterization of thermostable alpha-amylase by thermophilic Geobacillus stearothermophilus (2006), Biotechnol. J., 1, 850-857.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | EDTA | - |
Geobacillus stearothermophilus |  |
| 3.2.1.1 | Fe2+ | - |
Geobacillus stearothermophilus | |
| 3.2.1.1 | Fe3+ | - |
Geobacillus stearothermophilus | |
| 3.2.1.1 | Na+ | alpha-amylase in 1 M NaCl and 5 mM NaCl retains 73% and 43% of its original activity after 24 h at 4ºC, respectively | Geobacillus stearothermophilus | |
| 3.2.1.1 | Ni2+ | - |
Geobacillus stearothermophilus | |
| 3.2.1.1 | Zn2+ | - |
Geobacillus stearothermophilus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | Ca2+ | slightly improves enzymatic activity | Geobacillus stearothermophilus | |
| 3.2.1.1 | Cu2+ | slightly improves enzymatic activity | Geobacillus stearothermophilus | |
| 3.2.1.1 | Mg2+ | slightly improves enzymatic activity | Geobacillus stearothermophilus | |
| 3.2.1.1 | Mn2+ | slightly improves enzymatic activity | Geobacillus stearothermophilus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Geobacillus stearothermophilus | - |
- |
- |
| 3.2.1.1 | Geobacillus stearothermophilus JT2 | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | soluble starch + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
| 3.2.1.1 | soluble starch + H2O | - |
Geobacillus stearothermophilus JT2 | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 65 | - |
- |
Geobacillus stearothermophilus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 30 | 75 | 30°C: about 50% of maximal activity, 75°C: about 50% of maximal activity | Geobacillus stearothermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 7 | - |
- |
Geobacillus stearothermophilus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 6 | 13 | pH 6.0: about 35% of maximal activity, pH 13: about 45% of maximal activity | Geobacillus stearothermophilus |
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