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Literature summary extracted from

  • Erhardt, F.A.; Kuegler, J.; Chakravarthula, R.R.; Joerdening, H.J.
    Co-Immobilization of dextransucrase and dextranase for the facilitated synthesis of isomalto-oligosaccharides: Preparation, characterization and modeling (2008), Biotechnol. Bioeng., 100, 673-683.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.5 additional information co-immobilization of dextransucrase and dextranase on calcium alginate for the facilitated synthesis of isomalto-oligosaccharides, reaction scheme, method optimization, and modeling, overview Leuconostoc mesenteroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.11 additional information degree of enzyme inactivation declines with increasing ratio dextranase per dextranase adsorbate bentonite, at a ratio below 0.5: (w/w) only one half of the initial activity is retained Talaromyces aculeatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.5 additional information
-
 additional information kinetic modeling of fed-batch isomaltose production in a bi-enzymic system with immobolized enzymes, overview Leuconostoc mesenteroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.11 59000
-
 dextranase I, SDS-PAGE Talaromyces aculeatus
3.2.1.11 80000
-
 dextranase II, SDS-PAGE Talaromyces aculeatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.5 sucrose + dextran Leuconostoc mesenteroides glucose transfer, a step in production of isomaltose, overview D-fructose + ?
-
 ?
2.4.1.5 sucrose + dextran Leuconostoc mesenteroides NRRL B-512F glucose transfer, a step in production of isomaltose, overview D-fructose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc mesenteroides
-
-
-
2.4.1.5 Leuconostoc mesenteroides NRRL B-512F
-
-
-
3.2.1.11 Talaromyces aculeatus
-
 ATCC 10409
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.11 by SEC Talaromyces aculeatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.5 commercial preparation
-
 Leuconostoc mesenteroides
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.11 770
-
 dextranase I Talaromyces aculeatus
3.2.1.11 1670
-
 dextranase II Talaromyces aculeatus

Storage Stability

EC Number Storage Stability Organism
3.2.1.11 -20°C, dextranase II stable for 560 days Talaromyces aculeatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 sucrose + dextran glucose transfer, a step in production of isomaltose, overview Leuconostoc mesenteroides D-fructose + ?
-
 ?
2.4.1.5 sucrose + dextran glucose transfer Leuconostoc mesenteroides D-fructose + ?
-
 ?
2.4.1.5 sucrose + dextran glucose transfer, a step in production of isomaltose, overview Leuconostoc mesenteroides NRRL B-512F D-fructose + ?
-
 ?
2.4.1.5 sucrose + dextran glucose transfer Leuconostoc mesenteroides NRRL B-512F D-fructose + ?
-
 ?
3.2.1.11 dextran + H2O
-
 Talaromyces aculeatus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.5 DSR-S
-
 Leuconostoc mesenteroides
3.2.1.11 1,6-alpha-D-glucan-6-glucanohydrolase
-
 Talaromyces aculeatus
3.2.1.11 dextranase I
-
 Talaromyces aculeatus
3.2.1.11 dextranase II
-
 Talaromyces aculeatus
3.2.1.11 endo-dextranase
-
 Talaromyces aculeatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 30
-
 assay at Leuconostoc mesenteroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.11 760
-
 Dextran dextranase I Talaromyces aculeatus
3.2.1.11 2230
-
 Dextran dextranase II Talaromyces aculeatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.4
-
 assay at Leuconostoc mesenteroides
3.2.1.11 4.6
-
 dextranase II Talaromyces aculeatus
3.2.1.11 5
-
 dextranase I Talaromyces aculeatus