Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Saino, H.; Mizutani, M.; Hiratake, J.; Sakata, K.
    Expression and biochemical characterization of beta-primeverosidase and application of beta-primeverosylamidine to affinity purification (2008), Biosci. Biotechnol. Biochem., 72, 376-383.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.149 full-length cDNA, including a putative N-terminal secretion signal sequence, cloned into the baculovirus expression plasmid, expressed in Sf9 cells or High-five cells Camellia sinensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.149 additional information not inhibited by beta-glucosylamidine Camellia sinensis
3.2.1.149 N-(2-phenylethanimidoyl)-6-O-beta-D-xylopyranosyl-beta-D-glucopyranosylamine strongly inhibits Camellia sinensis
3.2.1.149 N-[4-[(1-benzyl-2,5-dioxopyrrolidin-3-yl)sulfanyl]butanimidoyl]-6-O-beta-D-xylopyranosyl-beta-D-glucopyranosylamine potent inhibitor Camellia sinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.149 60000
-
 1 * 60000, SDS-PAGE Camellia sinensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.149 Camellia sinensis Q7X9A9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.149 by gel filtration and affinity chromatography using a primeverosylamidine column, 30fold purified Camellia sinensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.149 0.9
-
 crude enzyme Camellia sinensis
3.2.1.149 26.8
-
 30fold purified enzyme Camellia sinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.149 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside + H2O hydrolyzed with lower activity Camellia sinensis alpha-L-arabinopyranose + beta-D-glucopyranose
-
 ?
3.2.1.149 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O hydrolyzed with lower activity Camellia sinensis beta-D-apiofuranose + beta-D-glucopyranose
-
 ?
3.2.1.149 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + H2O hydrolyzed with highest activity Camellia sinensis beta-D-xylopyranose + beta-D-glucopyranose
-
 ?
3.2.1.149 6-O-beta-L-arabinopyranosyl-beta-D-glucopyranoside + H2O hydrolyzed with lower activity Camellia sinensis beta-D-arabinopyranose + beta-D-glucopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.149 ? 1 * 60000, SDS-PAGE Camellia sinensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.149 0.026
-
 N-[4-[(1-benzyl-2,5-dioxopyrrolidin-3-yl)sulfanyl]butanimidoyl]-6-O-beta-D-xylopyranosyl-beta-D-glucopyranosylamine
-
 Camellia sinensis
3.2.1.149 0.14
-
 N-(2-phenylethanimidoyl)-6-O-beta-D-xylopyranosyl-beta-D-glucopyranosylamine
-
 Camellia sinensis