EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | 2-phenyl-N-glucosyl-acetamidium bromide | - |
Rosa x damascena |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | 2-phenylethyl beta-D-glucopyranoside + H2O | Rosa x damascena | - |
2-phenylethanol + beta-D-glucose | 2-phenylethanol is a flower sent compound | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Rosa x damascena | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.21 | native enzyme 36fold from petals to homogeneity by ammonium sulfate fractionation, two steps of ion exchange chromatography, and ultrafiltration | Rosa x damascena |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.21 | flower | - |
Rosa x damascena | - |
3.2.1.21 | petal | - |
Rosa x damascena | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 1.1 | - |
purified enzyme | Rosa x damascena |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | (S)-citronellyl beta-D-glucopyranoside + H2O | high activity, no activity with (S)-citronellyl beta-D-galactopyranoside or (S)-citronellyl beta-D-xylopyranoside | Rosa x damascena | (S)-citronellol + beta-D-glucose | - |
? | |
3.2.1.21 | (Z)-3-hexenyl beta-D-glucopyranoside + H2O | high activity, no activity with (Z)-3-hexenyl beta-D-galactopyranoside or Z-3-hexenyl beta-D-xylopyranoside | Rosa x damascena | (Z)-3-hexenol + beta-D-glucose | - |
? | |
3.2.1.21 | 2-phenylethyl beta-D-glucopyranoside + H2O | - |
Rosa x damascena | 2-phenylethanol + beta-D-glucose | 2-phenylethanol is a flower sent compound | ? | |
3.2.1.21 | 2-phenylethyl beta-D-glucopyranoside + H2O | best substrate, poor activity with 2-phenylethyl beta-D-galactopyranoside, no activity with 2-phenylethyl beta-D-xylopyranoside | Rosa x damascena | 2-phenylethanol + beta-D-glucose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | poor activity with 4-nitrophenyl-beta-D-galactopyranoside, no activity with 4-nitrophenyl-beta-D-xylopyranoside | Rosa x damascena | 4-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.21 | additional information | the enzyme is highly specificic for beta-D-glucopyranosides, no activity with alpha-D-glucopyranosides | Rosa x damascena | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 35 | - |
- |
Rosa x damascena |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 45 | - |
purified enzyme, inactivation above | Rosa x damascena |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 6 | - |
- |
Rosa x damascena |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 5 | 7.5 | purified enzyme, most stable at pH 7.5, inactivation below pH 5.0 | Rosa x damascena |