EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.48 | beta-mercaptoethanol | 6% increase of activity at 20 mM | Metarhizium anisopliae | |
3.1.3.48 | dithiothreitol | 7.4% increase of activity at 20 mM | Metarhizium anisopliae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.48 | Ag+ | 16.6% inhibition at 2 mM | Metarhizium anisopliae | |
3.1.3.48 | Cu2+ | 32.2% inhibition at 2 mM | Metarhizium anisopliae | |
3.1.3.48 | Fe2+ | 34.5% inhibition at 2 mM | Metarhizium anisopliae | |
3.1.3.48 | molybdate | 92.2% inhibition at 5 mM | Metarhizium anisopliae | |
3.1.3.48 | additional information | EDTA, sodium tartrate, sodium fluoride, or okadaic acid have no effect on activity | Metarhizium anisopliae | |
3.1.3.48 | N-Methylmaleimide | 76.3% inhibition at 0.5 mM | Metarhizium anisopliae | |
3.1.3.48 | orthovanadate | 24.5% inhibition at 5 mM | Metarhizium anisopliae | |
3.1.3.48 | tungstate | 16.3% inhibition at 20 mM | Metarhizium anisopliae | |
3.1.3.48 | Zn2+ | 75.2% inhibition at 5 mM | Metarhizium anisopliae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.48 | Ba2+ | 42.5% increase of activity at 20 mM | Metarhizium anisopliae | |
3.1.3.48 | Mn2+ | 9.2% increase of activity at 20 mM | Metarhizium anisopliae | |
3.1.3.48 | additional information | Mg2+, Co2+, or Ca2+ have no effect on activity | Metarhizium anisopliae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.48 | 82500 | - |
SDS-PAGE | Metarhizium anisopliae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.48 | Metarhizium anisopliae | - |
- |
- |
3.1.3.48 | Metarhizium anisopliae CQMa102 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.48 | ConA-Sepharose 4B column chromatography, HighQ anion resin chromatography, and 25S cation resin column chromatography | Metarhizium anisopliae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.48 | 0.045 | - |
culture medium, using p-nitrophenyl phosphate as a substrate | Metarhizium anisopliae |
3.1.3.48 | 8.681 | - |
after 192.9fold purification, using p-nitrophenyl phosphate as a substrate | Metarhizium anisopliae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.48 | ATP + H2O | 9.5% activity compared to TRDIpYETDYYRK | Metarhizium anisopliae | ADP + phosphate | - |
? | |
3.1.3.48 | ATP + H2O | 9.5% activity compared to TRDIpYETDYYRK | Metarhizium anisopliae CQMa102 | ADP + phosphate | - |
? | |
3.1.3.48 | additional information | no activity against phytic acid, ADP, AMP, O-phospho-L-serine or O-phospho-L-threonine | Metarhizium anisopliae | ? | - |
? | |
3.1.3.48 | additional information | no activity against phytic acid, ADP, AMP, O-phospho-L-serine or O-phospho-L-threonine | Metarhizium anisopliae CQMa102 | ? | - |
? | |
3.1.3.48 | O-phospho-L-tyrosine + H2O | - |
Metarhizium anisopliae | L-tyrosine + phosphate | - |
? | |
3.1.3.48 | O-phospho-L-tyrosine + H2O | - |
Metarhizium anisopliae CQMa102 | L-tyrosine + phosphate | - |
? | |
3.1.3.48 | p-nitrophenyl phosphate + H2O | 60.3% activity compared to TRDIpYETDYYRK | Metarhizium anisopliae | p-nitrophenol + phosphate | - |
? | |
3.1.3.48 | p-nitrophenyl phosphate + H2O | 60.3% activity compared to TRDIpYETDYYRK | Metarhizium anisopliae CQMa102 | p-nitrophenol + phosphate | - |
? | |
3.1.3.48 | TRDIpYETDYYRK + H2O | 100% activity | Metarhizium anisopliae | TRDIYETDYYRK + phosphate | - |
? | |
3.1.3.48 | TRDIpYETDYYRK + H2O | 100% activity | Metarhizium anisopliae CQMa102 | TRDIYETDYYRK + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.48 | protein tyrosine phosphatase | - |
Metarhizium anisopliae |
3.1.3.48 | PTPase | - |
Metarhizium anisopliae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.48 | 75 | - |
- |
Metarhizium anisopliae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.48 | 30 | 70 | remains stable after 1 h at up to 70°C | Metarhizium anisopliae |
3.1.3.48 | 80 | - |
loses 31% activity after 10 min at 80°C | Metarhizium anisopliae |
3.1.3.48 | 90 | - |
complete loss of activity after 10 min at 90°C | Metarhizium anisopliae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.48 | 5.5 | - |
- |
Metarhizium anisopliae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.48 | 3 | 9.5 | at 30°C | Metarhizium anisopliae |
3.1.3.48 | 4.5 | 8 | stable at 70°C, but activity decreases markedly at pH below 4.5 or pH above 8.0 | Metarhizium anisopliae |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.3.48 | Metarhizium anisopliae | isoelectric focusing | - |
9.5 |