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Literature summary extracted from

  • Walters, M.J.; Srikannathasan, V.; McEwan, A.R.; Naismith, J.H.; Fierke, C.A.; Toone, E.J.
    Characterization and crystal structure of Escherichia coli KDPGal aldolase (2008), Bioorg. Med. Chem., 16, 710-720.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.14 T161A strongly reduced activity Escherichia coli
4.1.2.14 T161V strongly reduced activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.14 0.1
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate wild type enzyme, in 20 mM HEPES, pH 7.5, at 23°C Escherichia coli
4.1.2.14 0.2
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate mutant enzyme T161A, in 20 mM HEPES, pH 7.5, at 23°C Escherichia coli
4.1.2.14 0.3
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate mutant enzyme T161V, in 20 mM HEPES, pH 7.5, at 23°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.14 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.14 2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
 Escherichia coli pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.14 additional information 2-dehydro-3-deoxy-D-galactonate 6-phosphate is a weak substrate Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.14 2-keto-3-deoxy-6-phosphogluconate aldolase
-
 Escherichia coli
4.1.2.14 KDPG aldolase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.14 0.02
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate mutant enzyme T161V, in 20 mM HEPES, pH 7.5, at 23°C Escherichia coli
4.1.2.14 9.8
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate mutant enzyme T161A, in 20 mM HEPES, pH 7.5, at 23°C Escherichia coli
4.1.2.14 80
-
 2-dehydro-3-deoxy-D-gluconate 6-phosphate wild type enzyme, in 20 mM HEPES, pH 7.5, at 23°C Escherichia coli