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Literature summary extracted from

  • Hirose, J.; Ohsaki, T.; Nishimoto, N.; Matuoka, S.; Hiromoto, T.; Yoshida, T.; Minoura, T.; Iwamoto, H.; Fukasawa, K.M.
    Characterization of the metal-binding site in aminopeptidase B (2006), Biol. Pharm. Bull., 29, 2378-2382.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.6 expressed in Escherichia coli strain BL21 Rattus norvegicus
3.4.11.6 into the pGEX-4T-3 vector for expression in Escherichia coli BL21 cells Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.6 1,10-phenanthroline
-
 Rattus norvegicus
3.4.11.6 2,6-pyridinedicarboxylate
-
 Rattus norvegicus
3.4.11.6 bipyridine
-
 Rattus norvegicus
3.4.11.6 EDTA
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.6 0.000183
-
 L-Arg-2-naphthylamide metal-substituted enzyme form Co(II)-Ap-B, at pH 7.4 and 25°C Rattus norvegicus
3.4.11.6 0.000267
-
 L-Arg-2-naphthylamide recombinant Ap-B, at pH 7.4 and 25°C Rattus norvegicus
3.4.11.6 0.183
-
 Arg-beta-naphthylamide Co(II)-Ap-B Rattus norvegicus
3.4.11.6 0.267
-
 Arg-beta-naphthylamide Ap-B Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.6 Co2+
-
 Rattus norvegicus
3.4.11.6 Co2+ the metal-substituted derivative Co(II)-Ap-B contains almost 1 mol of cobalt(II) ions molecule Rattus norvegicus
3.4.11.6 Cu2+
-
 Rattus norvegicus
3.4.11.6 Cu2+ the metal-substituted derivative Cu(II)-Ap-B contains almost 1 mol of copper(II) ions per molecule Rattus norvegicus
3.4.11.6 Zn2+
-
 Rattus norvegicus
3.4.11.6 Zn2+ Ap-B contains 1 mol of Zn2+ ions per molecule Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.6 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.6 glutathione-Sepharose 4B column chromatography and Q-Sepharose column chromatography Rattus norvegicus
3.4.11.6 using a glutathione-Sepharose 4B column, cleaving of the fusion-enzyme by thrombin, using a benzamidine-Sepharose and a Q-Sepharose column Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.6 duodenum
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.6 0.1
-
 metal-substituted enzyme form Cu(II)-Ap-B, using L-Arg-2-naphthylamide as a substrate, at pH 7.4 and 25°C Rattus norvegicus
3.4.11.6 0.1
-
 Cu(II)-Ap-B Rattus norvegicus
3.4.11.6 9.9
-
 metal-substituted enzyme form Co(II)-Ap-B, using L-Arg-2-naphthylamide as a substrate, at pH 7.4 and 25°C Rattus norvegicus
3.4.11.6 9.9
-
 Co(II)-Ap-B Rattus norvegicus
3.4.11.6 12.5
-
 recombinant Ap-B, using L-Arg-2-naphthylamide as a substrate, at pH 7.4 and 25°C Rattus norvegicus
3.4.11.6 12.5
-
 Ap-B Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.6 Arg-beta-naphthylamide + H2O substrate used in the activity assay Rattus norvegicus L-arginine + 2-naphthylamine
-
 ?
3.4.11.6 L-Arg-2-naphthylamide + H2O
-
 Rattus norvegicus L-Arg + 2-naphthylamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.6 aminopeptidase B
-
 Rattus norvegicus
3.4.11.6 aminopeptidase-B
-
 Rattus norvegicus
3.4.11.6 Ap-B
-
 Rattus norvegicus
3.4.11.6 Co(II)-Ap-B metal-substituted derivative of Ap-B Rattus norvegicus
3.4.11.6 Cu(II)-Ap-B metal-substituted derivative of Ap-B Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.6 25
-
 activity assay Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.6 53.1
-
 L-Arg-2-naphthylamide recombinant Ap-B, at pH 7.4 and 25°C Rattus norvegicus
3.4.11.6 53.1
-
 Arg-beta-naphthylamide Ap-B Rattus norvegicus
3.4.11.6 69.47
-
 L-Arg-2-naphthylamide metal-substituted enzyme form Co(II)-Ap-B, at pH 7.4 and 25°C Rattus norvegicus
3.4.11.6 69.5
-
 Arg-beta-naphthylamide Co(II)-Ap-B Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.6 7.5
-
 activity assay Rattus norvegicus