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Literature summary extracted from

  • Pouckova, P.; Morbio, M.; Vottariello, F.; Laurents, D.V.; Matousek, J.; Soucek, J.; Gotte, G.; Donadelli, M.; Costanzo, C.; Libonati, M.
    Cytotoxicity of polyspermine-ribonuclease A and polyspermine-dimeric ribonuclease A (2007), Bioconjug. Chem., 18, 1946-1955.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.6.1.18 medicine cross-linking of enzyme or its covalently linked dimer to polyspermine using dimethyl suberimidate. The in vitro and in vivo cytotoxic activity of treated monomeric enzyme is not higher than that of free polyspermine, but dimeric suberimidate-treated enzyme proves to be a more efficinet antitumor agent both in vitro and in vivo Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.18 additional information cross-linking of enzyme or its covalently linked dimer to polyspermine using dimethyl suberimidate. The in vitro and in vivo cytotoxic activity of treated monomeric enzyme is not higher than that of free polyspermine, but dimeric suberimidate-treated enzyme proves to be a more efficient antitumor agent both in vitro and in vivo Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Bos taurus
-
 Type XII-A, commercial preparation
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.18 pancreas
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.18 1.14
-
 substrate poly(A)poly(U), dimethyl suberimidate-treated, monomeric RNase A, pH 7.0, 25°C Bos taurus
4.6.1.18 1.55
-
 substrate poly(A)poly(U), native, monomeric RNase A, pH 7.0, 25°C Bos taurus
4.6.1.18 2.22
-
 substrate poly(A)poly(U), cross-linked RNase A dimer, pH 7.0, 25°C Bos taurus
4.6.1.18 12.15
-
 substrate poly(A)poly(U), domain-swapped RNase A C-dimer pH 7.0, 25°C Bos taurus
4.6.1.18 12.67
-
 substrate yeast RNA, cross-linked RNase A dimer, pH 7.0, 25°C Bos taurus
4.6.1.18 13.33
-
 substrate yeast RNA, cross-linked RNase A dimer, treated with dimethyl suberimidate, pH 7.0, 25°C Bos taurus
4.6.1.18 25.21
-
 substrate poly(A)poly(U), cross-linked RNase A dimer, treated with dimethyl suberimidate, pH 7.0, 25°C Bos taurus
4.6.1.18 49.33
-
 substrate yeast RNA, dimethyl suberimidate-treated, monomeric RNase A, pH 7.0, 25°C Bos taurus
4.6.1.18 53.46
-
 substrate yeast RNA, domain-swapped RNase A C-dimer pH 7.0, 25°C Bos taurus
4.6.1.18 84.55
-
 substrate yeast RNA, native, monomeric RNase A, pH 7.0, 25°C Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.18 poly(A)poly(U) + H2O
-
 Bos taurus ?
-
 ?
4.6.1.18 RNA + H2O substrate yeast RNA Bos taurus 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?