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Literature summary extracted from

  • Cherif, S.; Fendri, A.; Miled, N.; Trabelsi, H.; Mejdoub, H.; Gargouri, Y.
    Crab digestive lipase acting at high temperature: purification and biochemical characterization (2007), Biochimie, 89, 1012-1018.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 sodium deoxycholate the enzyme is inhibited by bile salts Homarus americanus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 65000
-
 x * 65000, SDS-PAGE Homarus americanus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Homarus americanus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 native enzyme 165fold from hepatopancreas by heat treatment, ammonium sulfate fractionation, gel filtration, anion exchange chromatography, and a second gel filtration step Homarus americanus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 digestive gland
-
 Homarus americanus
-
3.1.1.3 hepatopancreas
-
 Homarus americanus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 500
-
 purified native enzyme Homarus americanus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 olive oil + H2O
-
 Homarus americanus ?
-
 ?
3.1.1.3 tributyrin + H2O
-
 Homarus americanus dibutyrin + butyrate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 65000, SDS-PAGE Homarus americanus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 CDL
-
 Homarus americanus
3.1.1.3 crab digestive lipase
-
 Homarus americanus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 60
-
-
 Homarus americanus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 37 60 10% of maximal activity at 37°C, maximal activity at 60°C, complete inactivation at 65°C Homarus americanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
 assay at Homarus americanus