BRENDA - Enzyme Database

Interactions of enolase isoforms with tubulin and microtubules during myogenesis

Keller, A.; Peltzer, J.; Carpentier, G.; Horvath, I.; Olah, J.; Duchesnay, A.; Orosz, F.; Ovadi, J.; Biochim. Biophys. Acta 1770, 919-926 (2007)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
2-phosphoglycerate
presence of 0.8 mM 2-phosphoglycerate abolished the binding of beta,beta-enolase to tubulin, kinetics shown
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytosol
cytosolic fractions of myoblasts and myotubes, microtubule-association of alpha,alpha-enolase in undifferentiated myoblasts and in myotubes, also nuclear localization in myoblasts but not in myotubes determined, role of enolases in cytoskeletal dynamics during myoblast differentiation assumed; nuclear localization in myoblasts not detected, direct interaction with microtubules and tubulin during myoblast differentiation identified, role in cytoskeletal dynamics during myoblast differentiation assumed
Mus musculus
5829
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Mus musculus
-
phosphoenolpyruvate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Mus musculus
P17182
alpha,alpha-enolase
-
4.2.1.11
Mus musculus
P21550
beta,beta-enolase
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
for localization studies, immunochemistry and confocal microscopy; gel filtration, for localization studies and tubulin-binding kinetics
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
skeletal muscle
alpha,beta heterodimer and beta,beta homodimer in striated muscle, interaction with tubulin during differentiation of muscle satellite cells but not in undifferentiated myoblasts, immobilized beta,beta-enolase interacts with tubulin in vitro; satellite cell differentiation, alpha,alpha-enolase highly expressed during early ontogenesis, concentration of alpha,alpha-enolase in skeletal muscle varies from 0.1 mM to 0.01 mM until differentiation, binding affinity to tubulin is about 0.2 microM, alpha,beta heteroassociation with tubulin in muscle under physiological conditions
Mus musculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
enzyme activity measured with the natural substrate 2-phospho-D-glycerate, conversion of reaction products determined by spectroscopy, kinetics of binding studies to tubulin by ELISA and surface plasmon resonance; enzyme activity tested, kinetics of binding studies to tubulin estimated by ELISA and surface plasmon resonance, association of beta,beta enolase to microtubules in differentiating myotubes but not in myoblasts
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
678431
Mus musculus
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
interaction studies between alpha,alpha enolase and tubulin, co-localization studies with microtubules
678431
Mus musculus
phosphoenolpyruvate + H2O
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.11
37
-
enzyme activity assay at; enzyme activity assay at
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7.5
-
enzyme activity assay at; enzyme activity assay at
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
2-phosphoglycerate
presence of 0.8 mM 2-phosphoglycerate abolished the binding of beta,beta-enolase to tubulin, kinetics shown
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytosol
cytosolic fractions of myoblasts and myotubes, microtubule-association of alpha,alpha-enolase in undifferentiated myoblasts and in myotubes, also nuclear localization in myoblasts but not in myotubes determined, role of enolases in cytoskeletal dynamics during myoblast differentiation assumed
Mus musculus
5829
-
4.2.1.11
cytosol
nuclear localization in myoblasts not detected, direct interaction with microtubules and tubulin during myoblast differentiation identified, role in cytoskeletal dynamics during myoblast differentiation assumed
Mus musculus
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Mus musculus
-
phosphoenolpyruvate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
for localization studies, immunochemistry and confocal microscopy
Mus musculus
4.2.1.11
gel filtration, for localization studies and tubulin-binding kinetics
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
skeletal muscle
satellite cell differentiation, alpha,alpha-enolase highly expressed during early ontogenesis, concentration of alpha,alpha-enolase in skeletal muscle varies from 0.1 mM to 0.01 mM until differentiation, binding affinity to tubulin is about 0.2 microM, alpha,beta heteroassociation with tubulin in muscle under physiological conditions
Mus musculus
-
4.2.1.11
skeletal muscle
alpha,beta heterodimer and beta,beta homodimer in striated muscle, interaction with tubulin during differentiation of muscle satellite cells but not in undifferentiated myoblasts, immobilized beta,beta-enolase interacts with tubulin in vitro
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
enzyme activity measured with the natural substrate 2-phospho-D-glycerate, conversion of reaction products determined by spectroscopy, kinetics of binding studies to tubulin by ELISA and surface plasmon resonance
Mus musculus
4.2.1.11
additional information
-
enzyme activity tested, kinetics of binding studies to tubulin estimated by ELISA and surface plasmon resonance, association of beta,beta enolase to microtubules in differentiating myotubes but not in myoblasts
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
678431
Mus musculus
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
interaction studies between alpha,alpha enolase and tubulin, co-localization studies with microtubules
678431
Mus musculus
phosphoenolpyruvate + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.11
37
-
enzyme activity assay at
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
7.5
-
enzyme activity assay at
Mus musculus