Any feedback?
Literature summary extracted from
- Mutation of Lys242 allows DELTA3-DELTA2-enoyl-CoA isomerase to acquire enoyl-CoA hydratase activity (2006), Biochim. Biophys. Acta, 1760, 1874-1883.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.3.8 | - |
Rattus norvegicus |
| 5.3.3.8 | expression in Escherichia coli | Rattus norvegicus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.3.3.8 | D149E | decrease in catalytic activity | Rattus norvegicus |
| 5.3.3.8 | D149E | turnover number for trans-3-hexenoyl-CoA is 2.3fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme | Rattus norvegicus |
| 5.3.3.8 | K242C | decrease in catalytic activity, mutants shows additional hydratase activity on 2-hexenoyl-CoA with Vmax of 1.9 micromol per min and mg | Rattus norvegicus |
| 5.3.3.8 | K242C | turnover number for trans-3-hexenoyl-CoA is 14.5fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme | Rattus norvegicus |
| 5.3.3.8 | K242C | turnover number for trans-3-hexenoyl-CoA is 160 fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme. Mutation allows DELTA3-DELTA2-enoyl-CoA isomerase to acquire enoyl-CoA hydratase activity | Rattus norvegicus |
| 5.3.3.8 | K242D | decrease in catalytic activity, mutants shows slight additional hydratase activity on 2-hexenoyl-CoA with Vmax of 0.026 micromol per min and mg | Rattus norvegicus |
| 5.3.3.8 | K242D | turnover number for trans-3-hexenoyl-CoA is 12308fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme | Rattus norvegicus |
| 5.3.3.8 | K242D | turnover number for trans-3-hexenoyl-CoA is 2667fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme | Rattus norvegicus |
| 5.3.3.8 | K242F | decrease in catalytic activity | Rattus norvegicus |
| 5.3.3.8 | K242F | turnover number for trans-3-hexenoyl-CoA is 1143fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme | Rattus norvegicus |
| 5.3.3.8 | K242R | decrease in catalytic activity | Rattus norvegicus |
| 5.3.3.8 | K242R | turnover number for trans-3-hexenoyl-CoA is 3.2fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme | Rattus norvegicus |
| 5.3.3.8 | K242T | decrease in catalytic activity, mutants shows additional hydratase activity on 2-hexenoyl-CoA with Vmax of 0.041 micromol per min and mg | Rattus norvegicus |
| 5.3.3.8 | K242T | turnover number for trans-3-hexenoyl-CoA is 7619fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme | Rattus norvegicus |
| 5.3.3.8 | K242T | turnover number for trans-3-hexenoyl-CoA is 842fold lower than wild-type value, Km-value for trans-3-hexenoyl-CoA is fold higher than wild-type enzyme | Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.3.8 | 0.00062 | - |
trans-2-hexenoyl-CoA | pH 8, hydratase activity, mutant enzyme K242T | Rattus norvegicus |  |
| 5.3.3.8 | 0.0026 | - |
trans-2-hexenoyl-CoA | pH 8, hydratase activity, mutant enzyme K242D | Rattus norvegicus | |
| 5.3.3.8 | 0.03 | - |
trans-3-hexenoyl-CoA | 25°C, pH 7.5, mutant enzyme K242D | Rattus norvegicus | |
| 5.3.3.8 | 0.03 | - |
3-trans-hexenoyl-CoA | mutant K242D, pH 8.0 | Rattus norvegicus | |
| 5.3.3.8 | 0.048 | - |
trans-3-hexenoyl-CoA | 25°C, pH 7.5, wild-type enzyme | Rattus norvegicus | |
| 5.3.3.8 | 0.048 | - |
3-trans-hexenoyl-CoA | wild-type, pH 8.0 | Rattus norvegicus | |
| 5.3.3.8 | 0.076 | - |
trans-3-hexenoyl-CoA | 25°C, pH 7.5, mutant enzyme K242T | Rattus norvegicus | |
| 5.3.3.8 | 0.076 | - |
3-trans-hexenoyl-CoA | mutant K242T, pH 8.0 | Rattus norvegicus | |
| 5.3.3.8 | 0.08 | - |
trans-3-hexenoyl-CoA | 25°C, pH 7.5, mutant enzyme D149E | Rattus norvegicus | |
| 5.3.3.8 | 0.08 | - |
3-trans-hexenoyl-CoA | mutant D149E, pH 8.0 | Rattus norvegicus | |
| 5.3.3.8 | 0.089 | - |
trans-3-hexenoyl-CoA | 25°C, pH 7.5, mutant enzyme K242R | Rattus norvegicus | |
| 5.3.3.8 | 0.089 | - |
3-trans-hexenoyl-CoA | mutant K242R, pH 8.0 | Rattus norvegicus | |
| 5.3.3.8 | 0.1 | - |
trans-3-hexenoyl-CoA | 25°C, pH 7.5, mutant enzyme K242F | Rattus norvegicus | |
| 5.3.3.8 | 0.1 | - |
3-trans-hexenoyl-CoA | mutant K242F, pH 8.0 | Rattus norvegicus | |
| 5.3.3.8 | 0.12 | - |
trans-3-hexenoyl-CoA | 25°C, pH 7.5, mutant enzyme K242C | Rattus norvegicus | |
| 5.3.3.8 | 0.12 | - |
3-trans-hexenoyl-CoA | mutant K242C, pH 8.0 | Rattus norvegicus | |
| 5.3.3.8 | 0.31 | - |
trans-2-hexenoyl-CoA | pH 8, hydratase activity, mutant enzyme K242C | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.3.3.8 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.3.8 | additional information | Rattus norvegicus | one of the key enzymes involved in fatty acid oxidation | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.3.8 | Rattus norvegicus | - |
- |
- |
| 5.3.3.8 | Rattus norvegicus | P23965 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.3.8 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.3.3.8 | liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.3.8 | 3-trans-Hexenoyl-CoA | - |
Rattus norvegicus | 2-trans-Hexenoyl-CoA | - |
? | |
| 5.3.3.8 | additional information | one of the key enzymes involved in fatty acid oxidation | Rattus norvegicus | ? | - |
? | |
| 5.3.3.8 | trans-2-hexenoyl-CoA + H2O | K242C mutation allows Delta3-Delta2-enoyl-CoA isomerase to acquire enoyl-CoA hydratase activity | Rattus norvegicus | 3-hydroxyhexanoyl-CoA | - |
? | |
| 5.3.3.8 | trans-3-hexenoyl-CoA | - |
Rattus norvegicus | trans-2-hexenoyl-CoA | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.3.8 | Delta3-Delta2-enoyl-CoA isomerase | - |
Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.17 | 180 | - |
trans-2-hexenoyl-CoA | pH 8 | Bos taurus | |
| 5.3.3.8 | 0.013 | - |
trans-2-hexenoyl-CoA | pH 8, hydratase activity, mutant enzyme K242D | Rattus norvegicus | |
| 5.3.3.8 | 0.021 | - |
trans-2-hexenoyl-CoA | pH 8, hydratase activity, mutant enzyme K242T | Rattus norvegicus | |
| 5.3.3.8 | 0.06 | - |
trans-3-hexenoyl-CoA | 25°C, pH 7.5, mutant enzyme K242D | Rattus norvegicus | |
| 5.3.3.8 | 0.06 | - |
3-trans-hexenoyl-CoA | mutant K242D, pH 8.0 | Rattus norvegicus | |
| 5.3.3.8 | 0.14 | - |
trans-3-hexenoyl-CoA | 25°C, pH 7.5, mutant enzyme K242F | Rattus norvegicus | |
| 5.3.3.8 | 0.14 | - |
3-trans-hexenoyl-CoA | mutant K242F, pH 8.0 | Rattus norvegicus | |
| 5.3.3.8 | 0.19 | - |
trans-3-hexenoyl-CoA | 25°C, pH 7.5, mutant enzyme K242T | Rattus norvegicus | |
| 5.3.3.8 | 0.19 | - |
3-trans-hexenoyl-CoA | mutant K242T, pH 8.0 | Rattus norvegicus | |
| 5.3.3.8 | 1 | - |
trans-2-hexenoyl-CoA | pH 8, hydratase activity, mutant enzyme K242C | Rattus norvegicus | |
| 5.3.3.8 | 11 | - |
trans-3-hexenoyl-CoA | 25°C, pH 7.5, mutant enzyme K242C | Rattus norvegicus | |
| 5.3.3.8 | 11 | - |
3-trans-hexenoyl-CoA | mutant K242C, pH 8.0 | Rattus norvegicus | |
| 5.3.3.8 | 50 | - |
trans-3-hexenoyl-CoA | 25°C, pH 7.5, mutant enzyme K242R | Rattus norvegicus | |
| 5.3.3.8 | 50 | - |
3-trans-hexenoyl-CoA | mutant K242R, pH 8.0 | Rattus norvegicus | |
| 5.3.3.8 | 70 | - |
trans-3-hexenoyl-CoA | 25°C, pH 7.5, mutant enzyme D149E | Rattus norvegicus | |
| 5.3.3.8 | 70 | - |
3-trans-hexenoyl-CoA | mutant D149E, pH 8.0 | Rattus norvegicus | |
| 5.3.3.8 | 160 | - |
3-trans-hexenoyl-CoA | wild-type, pH 8.0 | Rattus norvegicus | |
| 5.3.3.8 | 160 | - |
trans-3-hexenoyl-CoA | 25°C, pH 7.5, wild-type enzyme | Rattus norvegicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
