Literature summary extracted from
Hoggins, M.; Dailey, H.A.; Hunter, C.N.; Reid, J.D.
Direct measurement of metal ion chelation in the active site of human ferrochelatase (2007), Biochemistry, 46, 8121-8127.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.98.1.1 |
recombinant human ferrochelatase (R115L) expressed in Escherichia coli JM109 cells |
Homo sapiens |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.98.1.1 |
R115L |
no impact on enzyme activity, exhibits a stoichiometric burst of product formation and substrate consumption, consistent with a rate-determining step following metal ion chelation |
Homo sapiens |
4.98.1.1 |
R115L/E343D |
exhibits similar hyperbolic concentration dependencies on porphyrin and ferrous iron concentrations, characteristic two-step mechanism with rapid substrate binding followed by a slower step of metal ion chelation, 7fold decrease in kcat. Mutation primarily affects events occurring after metal ion chelation |
Homo sapiens |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.98.1.1 |
Fe2+ |
metal ion chelation, with a rate constant of 0.96 s-1, is ca. 10times faster than the rate-determining step in the steady state |
Homo sapiens |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.98.1.1 |
Homo sapiens |
P22830 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.98.1.1 |
by gel filtration |
Homo sapiens |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.98.1.1 |
deuteroporphyrin + Fe2+ |
- |
Homo sapiens |
deuteroheme + 2 H+ |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.98.1.1 |
protoporphyrin IX ferrochelatase |
- |
Homo sapiens |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
4.98.1.1 |
0.1 |
- |
Fe2+ |
- |
Homo sapiens |
|