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Literature summary extracted from

  • Andersen, J.F.; Ribeiro, J.M.
    A secreted salivary inositol polyphosphate 5-phosphatase from a blood-feeding insect: allosteric activation by soluble phosphoinositides and phosphatidylserine (2006), Biochemistry, 45, 5450-5457.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.36 1,2-dioctanoylphosphatidylinositol 4-phosphate hydrolysis of D-myo-phosphatidylinositol 4,5-bisphosphate and D-myo-phosphatidylinositol 3,4,5-trisphosphate is stimulated up to 100fold Rhodnius prolixus
3.1.3.36 dioctanoylphosphatidylserine
-
 Rhodnius prolixus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.36 IPPRp is cloned into the pET17b expression vector and moved into Escherichia coli strain BL21(DE3)pLys S for protein production Rhodnius prolixus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.36 Ca2+ enzyme is inactive in the presence of 1 mM Ca2+ Rhodnius prolixus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.36 additional information
-
 additional information with soluble diC8 D-myo-phosphatidylinositol 4,5-bisphosphate as a substrate, sigmoidal kinetics are observed, suggesting the presence of allosteric activation sites Rhodnius prolixus
3.1.3.36 0.421
-
 1D-myo-inositol 1,4,5-trisphosphate
-
 Rhodnius prolixus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.36 Mg2+
-
 Rhodnius prolixus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.36 36500
-
 deduced from cDNA Rhodnius prolixus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.36 Rhodnius prolixus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.36 purification by gel filtration chromatography on Sephacryl S-100 followed by cation exchange chromatography on SP-Sepharose Rhodnius prolixus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.36 saliva
-
 Rhodnius prolixus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.36 1D-myo-inositol 1,4,5-trisphosphate + H2O
-
 Rhodnius prolixus 1D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
3.1.3.36 D-myo-phosphatidylinositol 3,4,5-trisphosphate
-
 Rhodnius prolixus D-myo-phosphatidylinositol 3,4-bisphosphate + phosphate
-
 ?
3.1.3.36 D-myo-phosphatidylinositol 4,5-bisphosphate D-myo-phosphatidylinositol 4,5-bisphosphate is a much better substrate than D-myo-phosphatidylinositol 3,4,5-trisphosphate Rhodnius prolixus D-myo-phosphatidylinositol 4-phosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.36 inositol polyphosphate 5-phosphatase
-
 Rhodnius prolixus
3.1.3.36 IPPRp
-
 Rhodnius prolixus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.36 30
-
 assay at Rhodnius prolixus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.36 7.4
-
 assay at Rhodnius prolixus