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Literature summary extracted from

  • Roy, A.; Das, B.B.; Ganguly, A.; Bosedasgupta, S.; Khalkho, N.V.; Pal, C.; Dey, S.; Giri, V.S.; Jaisankar, P.; Dey, S.; Majumder, H.K.
    An insight into the mechanism of inhibition of unusual bi-subunit topoisomerase I from Leishmania donovani by 3,3-diindolylmethane, a novel DNA topoisomerase I poison with a strong binding affinity to enzyme (2008), Biochem. J., 409, 611-622.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.6.2.1 drug development 3,3’-diindolylmethane is a novel Leishmania donovani topoisomerase I poison which acts as a noncompetitive inhibitor. So this intriguing dietary component might be exploited for therapeutic development against leishmaniasis Leishmania donovani

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.1 Escherichia coli BL21 (DE3) Leishmania donovani

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.1 3,3'-diindolylmethane (DIM) novel DNA topoisomerase I poison, which is a class I noncompetitive inhibitor, strongly inhibits Leishmania donovani topoisomerase I and stabilizes topoisomerase I-DNA cleavable complex Leishmania donovani
5.6.2.1 camptothecin most widely studied inhibitor of type IB topoisomerase Leishmania donovani
5.6.2.1 dihydrobetulinic acid catalytic inhibitor of topoisomerase I, inhibits the suicidal cleavage reaction Leishmania donovani

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.1 Leishmania donovani
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.1
-
 Leishmania donovani

Subunits

EC Number Subunits Comment Organism
5.6.2.1 heterodimer DNA topoisomerase I of kinetoplastid protozoan parasite Leishmania donovani is an unusual bi-subunit enzyme. To find out the differential affinity of 3,3’-diindolylmethane towards the large subunit (LdTOP1L) and small subunit (LdTOP1S) of Leishmania donovani topoisomerase I, performed the binding of individual subunits with 3,3’-diindolylmethane by equilibrium dialysis experiment Leishmania donovani

Synonyms

EC Number Synonyms Comment Organism
5.6.2.1 bi-subunit topoisomerase I
-
 Leishmania donovani
5.6.2.1 DNA topoisomerase
-
 Leishmania donovani
5.6.2.1 LdTOP1LS
-
 Leishmania donovani
5.6.2.1 Topoisomerase I
-
 Leishmania donovani

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.6.2.1 0.0012
-
 potent inhibitor of Leishmania donovani topoisomerase I Leishmania donovani 3,3'-diindolylmethane