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Literature summary extracted from

  • Suzuki, T.; Hara, I.; Nakano, M.; Shigeta, M.; Nakagawa, T.; Kondo, A.; Funakoshi, Y.; Taniguchi, N.
    Man2C1, an alpha-mannosidase, is involved in the trimming of free oligosaccharides in the cytosol (2006), Biochem. J., 400, 33-41.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 deoxymannojirimycin less effective compared with swainsonine, concentration of 100 microM Homo sapiens
3.2.1.24 kifunensine little inhibitory effect, concentration of 10 microM Homo sapiens
3.2.1.24 additional information down-regulation of Man2C1 activity by a small interfering RNA drastically change amount and structure of oligosaccharides accumulating in the cytosol, demonstrating that Man2C1 is involved in free oligosaccharide processing in the cytosol Homo sapiens
3.2.1.24 small interfering RNA siRNA designed to specifically inhibit Man2C1 gene expression, alpha-mannosidase activity is compromised in siRNA-treated cells. Determination of alteration of siRNA treatment, glycans recovered from membrane glycoproteins are fluorescence-labelled and analysed by HPLC Homo sapiens
3.2.1.24 swainsonine concentration of 100 microM Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.24 cytosol examination of subcellular localization of Man2C1, using FLAG-tagging at the N-terminus for detection Homo sapiens 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.24 Co2+ enhances acivity, alpha-mannosidase in absence of Co2+ only converts mannosyl(9)-N-acetyl-glucosamine into mannosyl(8)-N-acetyl-glucosamine, in presence of Co2+, alpha-mannosidase yields mannosyl(5)-N-acetyl-glucosamine Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Homo sapiens Q9NTJ4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 FLAG–tagged Man2C1 from cytosolic fraction of HEK-293 cells, analysed by silver staining Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 4-nitrophenyl-alpha-D-mannoside + H2O pNP-alpha-Man, used for enzyme activity test Homo sapiens 4-nitrophenol + alpha-D-mannose
-
 ?
3.2.1.24 mannosyl(5)-N-acetyl-glucosamine-pyridylamino + H2O
-
 Homo sapiens mannosyl(4)-N-acetyl-glucosamine-pyridylamino + mannosyl(3)-N-acetyl-glucosamine-pyridylamino occurrence of mannosyl(4)-N-acetyl-glucosamine-pyridylamino (32%) and mannosyl(3)-N-acetyl-glucosamine-pyridylamino (8.1%) are observed only in the presence of Co2+ ?
3.2.1.24 mannosyl(9)-N-acetyl-glucosamine + 4 H2O in presence of Co2+ Homo sapiens mannosyl(5)-N-acetyl-glucosamine + 4 alpha-D-mannose
-
 ?
3.2.1.24 mannosyl(9)-N-acetyl-glucosamine + H2O absence of Co2+ Homo sapiens mannosyl(8)-N-acetyl-glucosamine + alpha-D-mannose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.24 alpha-mannosidase
-
 Homo sapiens
3.2.1.24 ER-mannosidase II
-
 Homo sapiens
3.2.1.24 Man2C1
-
 Homo sapiens
3.2.1.24 Man2C1 alpha-mannosidase
-
 Homo sapiens
3.2.1.24 neutral/cytosol mannosidase
-
 Homo sapiens