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Literature summary extracted from

  • Heck, J.X.; Soares, L.H.; Hertz, P.F.; Ayub, M.A.
    Purification and properties of a xylanase produced by Bacillus circulans BL53 on solid-state cultivation (2006), Biochem. Eng. J., 32, 179-184.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 beta-mercaptoethanol 52.66% increase of activity at 2 mM Niallia circulans
3.2.1.8 dithiothreitol 11.6% increase of activity at 2 mM Niallia circulans
3.2.1.8 DMSO 6.58% increase of activity at 2 mM Niallia circulans
3.2.1.8 EDTA 14.48% increase of activity at 2 mM Niallia circulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ca2+ 95.39% residual activity at 2 mM Niallia circulans
3.2.1.8 Cu2+ 91.44% residual activity at 2 mM Niallia circulans
3.2.1.8 Fe2+ 81.9% residual activity at 2 mM Niallia circulans
3.2.1.8 Hg2+ no activity at 2 mM Niallia circulans
3.2.1.8 Mg2+ 82.55% residual activity at 2 mM Niallia circulans
3.2.1.8 Na+ 92.1% residual activity at 2 mM Niallia circulans
3.2.1.8 Pb2+ 78.93% residual activity at 2 mM Niallia circulans
3.2.1.8 polyhexamethylbiguanid 77.29% residual activity at 2 mM Niallia circulans
3.2.1.8 SDS 37.46% residual activity at 2 mM Niallia circulans
3.2.1.8 Zn2+ 91.11% residual activity at 2 mM Niallia circulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Co2+ 51.67% increase of activity at 2 mM Niallia circulans
3.2.1.8 Mn2+ 61.88% increase of activity at 2 mM Niallia circulans
3.2.1.8 NH4+ 9.21% increase of activity at 2 mM Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 38000
-
 SDS-PAGE Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Niallia circulans
-
-
-
3.2.1.8 Niallia circulans BL53
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 ammonium sulfate saturation, CM-Sepharose FF column chromatography, and Sephacryl S-200 HR gel filtration Niallia circulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 0.087
-
 crude extract, using birchwood xylan in 100 mM sodium acetate buffer pH 5.0 as the substrate Niallia circulans
3.2.1.8 37.22
-
 after 427.83fold purification, using birchwood xylan in 100 mM sodium acetate buffer pH 5.0 as the substrate Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birchwood xylan + H2O
-
 Niallia circulans D-xylose + ?
-
 ?
3.2.1.8 birchwood xylan + H2O
-
 Niallia circulans BL53 D-xylose + ?
-
 ?
3.2.1.8 grape skin + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.8 grape skin + H2O
-
 Niallia circulans BL53 ?
-
 ?
3.2.1.8 rice straw + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.8 rice straw + H2O
-
 Niallia circulans BL53 ?
-
 ?
3.2.1.8 soybean fiber + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.8 soybean fiber + H2O
-
 Niallia circulans BL53 ?
-
 ?
3.2.1.8 soybean hull + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.8 soybean hull + H2O
-
 Niallia circulans BL53 ?
-
 ?
3.2.1.8 sugar cane bagasse + H2O
-
 Niallia circulans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 monomer 1 * 38000, SDS-PAGE Niallia circulans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 1,4-beta-xylan xylanohydrolase
-
 Niallia circulans
3.2.1.8 xylanase
-
 Niallia circulans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 40 80
-
 Niallia circulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 5 8
-
 Niallia circulans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Niallia circulans isoelectric focusing
-
 8.8