Literature summary extracted from

Henz, S.L.; Fuerstenau, C.R.; Chiarelli, R.A.; Sarkis, J.J.
Kinetic and biochemical characterization of an ecto-nucleotide pyrophosphatase/phosphodiesterase (EC 3.1.4.1) in cells cultured from submandibular salivary glands of rats (2007), Arch. Oral Biol., 52, 916-923.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.1.4.1	AMP	0.5 mM, 70% residual activity	Rattus norvegicus
3.1.4.1	cAMP	3 mM, 86% residual activity	Rattus norvegicus
3.1.4.1	EDTA	complete inhibition, addition of EDTA plus Ca2+ or Mn2+ results in partial inhibition	Rattus norvegicus
3.1.4.1	additional information	not inhibitory: ouabain, N-ethylmaleimide, levamisole, sodium azide	Rattus norvegicus
3.1.4.1	phenylalanine	5 mM, 87% residual activity	Rattus norvegicus
3.1.4.1	suramin	0.25 mM, 87% residual activity	Rattus norvegicus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.1.4.1	0.281	-	p-nitrophenyl 5'-thymidine monophosphate	pH 7.4, 37°C	Rattus norvegicus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.1.4.1	Ca2+	or Mg2+, basal level required, no significant increase in activity by addition of 0.1 mM Ca2+ or Mg2+	Rattus norvegicus
3.1.4.1	Mg2+	or Ca2+, basal level required, no significant increase in activity by addition of 0.1 mM Ca2+ or Mg2+	Rattus norvegicus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.4.1	Rattus norvegicus	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.1.4.1	salivary gland	primary cell culture of submandibular salivary gland, co-localization of enzyme with an ecto-ATP diphosphohydrolase and an ecto-5'-nucleotidase	Rattus norvegicus	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.4.1	p-nitrophenyl 5'-thymidine monophosphate + H2O	-	Rattus norvegicus	p-nitrophenol + 5'-thymidine monophosphate	-	?

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Release 2023.1 (January 2023)