BRENDA - Enzyme Database

Crystal structure of the yeast nicotinamidase Pnc1p

Hu, G.; Taylor, A.B.; McAlister-Henn, L.; Hart, P.J.; Arch. Biochem. Biophys. 461, 66-75 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.5.1.19
expression in Escherichia coli
Saccharomyces cerevisiae
Crystallization (Commentary)
EC Number
Crystallization (Commentary)
Organism
3.5.1.19
resolution 2.9 Angstrom
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.21
-
nicotinamide
about 3fold higher than with pyrazinamide as substrate
Saccharomyces cerevisiae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.19
Zn2+
-
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.5.1.19
nicotinamide + H2O
Saccharomyces cerevisiae
-
NH3 + nicotinic acid
-
-
?
3.5.1.19
pyrazinamide + H2O
Saccharomyces cerevisiae
-
NH3 + pyrazinoic acid
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.5.1.19
Saccharomyces cerevisiae
P53184
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.5.1.19
immobilized metal affinity chromatography
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.19
50.2
-
-
Saccharomyces cerevisiae
3.5.1.19
59.4
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.5.1.19
nicotinamide + H2O
-
677824
Saccharomyces cerevisiae
NH3 + nicotinic acid
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
677824
Saccharomyces cerevisiae
NH3 + pyrazinoic acid
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
3.5.1.19
nicotinamidase Pnc1p
-
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
expression in Escherichia coli
Saccharomyces cerevisiae
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.5.1.19
resolution 2.9 Angstrom
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.21
-
nicotinamide
about 3fold higher than with pyrazinamide as substrate
Saccharomyces cerevisiae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.19
Zn2+
-
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.5.1.19
nicotinamide + H2O
Saccharomyces cerevisiae
-
NH3 + nicotinic acid
-
-
?
3.5.1.19
pyrazinamide + H2O
Saccharomyces cerevisiae
-
NH3 + pyrazinoic acid
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
immobilized metal affinity chromatography
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.19
50.2
-
-
Saccharomyces cerevisiae
3.5.1.19
59.4
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.5.1.19
nicotinamide + H2O
-
677824
Saccharomyces cerevisiae
NH3 + nicotinic acid
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
677824
Saccharomyces cerevisiae
NH3 + pyrazinoic acid
-
-
-
?