BRENDA - Enzyme Database show

Crystal structure of the yeast nicotinamidase Pnc1p

Hu, G.; Taylor, A.B.; McAlister-Henn, L.; Hart, P.J.; Arch. Biochem. Biophys. 461, 66-75 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.1.19
expression in Escherichia coli
Saccharomyces cerevisiae
Crystallization (Commentary)
EC Number
Crystallization
Organism
3.5.1.19
resolution 2.9 Angstrom
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.21
-
nicotinamide
about 3fold higher than with pyrazinamide as substrate
Saccharomyces cerevisiae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.19
Zn2+
-
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
nicotinamide + H2O
Saccharomyces cerevisiae
-
NH3 + nicotinic acid
-
-
?
3.5.1.19
pyrazinamide + H2O
Saccharomyces cerevisiae
-
NH3 + pyrazinoic acid
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Saccharomyces cerevisiae
P53184
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.19
immobilized metal affinity chromatography
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.19
50.2
-
-
Saccharomyces cerevisiae
3.5.1.19
59.4
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
677824
Saccharomyces cerevisiae
NH3 + nicotinic acid
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
677824
Saccharomyces cerevisiae
NH3 + pyrazinoic acid
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
expression in Escherichia coli
Saccharomyces cerevisiae
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.5.1.19
resolution 2.9 Angstrom
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.19
0.21
-
nicotinamide
about 3fold higher than with pyrazinamide as substrate
Saccharomyces cerevisiae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.19
Zn2+
-
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
nicotinamide + H2O
Saccharomyces cerevisiae
-
NH3 + nicotinic acid
-
-
?
3.5.1.19
pyrazinamide + H2O
Saccharomyces cerevisiae
-
NH3 + pyrazinoic acid
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
immobilized metal affinity chromatography
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5.1.19
50.2
-
-
Saccharomyces cerevisiae
3.5.1.19
59.4
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
-
677824
Saccharomyces cerevisiae
NH3 + nicotinic acid
-
-
-
?
3.5.1.19
pyrazinamide + H2O
-
677824
Saccharomyces cerevisiae
NH3 + pyrazinoic acid
-
-
-
?