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Literature summary extracted from

  • Hirata, Y.; Inagaki, H.; Shimizu, T.; Li, Q.; Nagahara, N.; Minami, M.; Kawada, T.
    Expression of enzymatically active human granzyme 3 in Escherichia coli for analysis of its substrate specificity (2006), Arch. Biochem. Biophys., 446, 35-43.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B4 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.B4 0.6
-
 benzyloxycarbonyl-Arg p-nitrobenzyl ester
-
 Homo sapiens
3.4.21.B4 5.2
-
 benzyloxycarbonyl-L-Lys-thiobenzyl ester
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B4 Homo sapiens P49863
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B4 DE52 anion-exchange column chromatography, Q Sepharose column chromatography, hydroxylapatite column chromatography, and Sephadex G-75 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.B4 cytotoxic T-cell
-
 Homo sapiens
-
3.4.21.B4 natural killer cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.B4 0.0529
-
 cell extract, using benzyloxycarbonyl-Lys-thiobenzyl ester as a substrate, at 25°C Homo sapiens
3.4.21.B4 37.1
-
 after 1202.9fold purification, using benzyloxycarbonyl-Arg p-nitrobenzyl ester as a substrate, at 25°C Homo sapiens
3.4.21.B4 63.7
-
 after 1202.9fold purification, using benzyloxycarbonyl-Lys-thiobenzyl ester as a substrate, at 25°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B4 benzyloxycarbonyl-Arg p-nitrobenzyl ester + H2O
-
 Homo sapiens benzyloxycarbonyl-Arg + p-nitrobenzyl alcohol
-
 ?
3.4.21.B4 benzyloxycarbonyl-L-Lys-thiobenzyl ester + H2O
-
 Homo sapiens benzyloxycarbonyl-L-Lys + thiobenzyl alcohol
-
 ?
3.4.21.B4 Cbz-L-Arg-4-nitroanilide + H2O little hydrolyzed by Gr3 Homo sapiens Cbz-L-Arg + 4-nitroaniline
-
 ?
3.4.21.B4 Cbz-L-Arg-7-amido-4-methylcoumarin + H2O little hydrolyzed by Gr3 Homo sapiens Cbz-L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B4 Cbz-L-Lys-4-nitroanilide + H2O little hydrolyzed by Gr3 Homo sapiens Cbz-L-Lys + 4-nitroaniline
-
 ?
3.4.21.B4 fluorescence resonance energy transfer substrate-25Arg peptide + H2O most effectively hydrolyzes the substrate fluorescence resonance energy transfer substrate-25Arg peptide in the FRETS-25Xaa series tested Homo sapiens ?
-
 ?
3.4.21.B4 fluorescence resonance energy transfer substrate-25Lys peptide + H2O far less effectively cleaved than fluorescence resonance energy transfer substrate-25Arg peptide Homo sapiens ?
-
 ?
3.4.21.B4 additional information most efficiently hydrolyzes the carboxylic side of Phe-Tyr-Arg (P3-P2-P1) sequence Homo sapiens ?
-
 ?
3.4.21.B4 Z-Leu-Arg-Gly-Gly-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Z-Leu-Arg + Gly-Gly-7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B4 Gr3
-
 Homo sapiens
3.4.21.B4 granzyme 3
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B4 8.5 9
-
 Homo sapiens