Any feedback?
Literature summary extracted from
- Purification and characterization of an extracellular alpha-L-arabinosidase from a novel isolate Bacillus pumilus ARA and its over-expression in Escherichia coli (2008), Appl. Microbiol. Biotechnol., 78, 115-121.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.55 | overexpression in Escherichia coli | Bacillus pumilus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.55 | Cu2+ | 1 mM, 15% inhibition | Bacillus pumilus |  |
| 3.2.1.55 | Hg2+ | 1 mM, complete inhibition | Bacillus pumilus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.55 | 1.05 | - |
p-nitrophenyl alpha-L-arabinofuranoside | - |
Bacillus pumilus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.55 | extracellular | - |
Bacillus pumilus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.55 | Mn2+ | 1 mM, 1.8fold activation | Bacillus pumilus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.55 | Bacillus pumilus | Q2MJP5 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.55 | - |
Bacillus pumilus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.55 | culture medium | - |
Bacillus pumilus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.55 | additional information | the enzyme releases arabinose from oat spelt xylan. No activity is detected with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl alpha-D-xylopyranoside, and 4-nitrophenyl beta-D-xylopyranoside | Bacillus pumilus | ? | - |
? | |
| 3.2.1.55 | p-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Bacillus pumilus | p-nitrophenol + L-arabinofuranose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.55 | AraB | - |
Bacillus pumilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 60 | - |
- |
Bacillus pumilus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 45 | 75 | 45°C: about 70% of maximal activity, 75°C: about 65% of maximal activity, substrate: p-nitrophenyl alpha-L-arabinofuranoside | Bacillus pumilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 70 | - |
half-ife: 1 h | Bacillus pumilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 6.4 | - |
substrate: p-nitrophenyl alpha-L-arabinofuranoside | Bacillus pumilus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 5.2 | 7.6 | pH 5.2: about 60% of maximal activity, pH 7.6: about 60% of maximal activity, substrate: p-nitrophenyl alpha-L-arabinofuranoside | Bacillus pumilus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 5.2 | 7.6 | 65°C, 1 h, stable | Bacillus pumilus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
