Literature summary extracted from

  • de Wet, B.J.; Matthew, M.K.; Storbeck, K.; van Zyl, W.H.; Prior, B.A.
    Characterization of a family 54 alpha -L-arabinofuranosidase from Aureobasidium pullulans (2008), Appl. Microbiol. Biotechnol., 77, 975-983.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 expression in Saccharomyces cerevisiae Aureobasidium pullulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 arabinose noncompetitive inhibitor, 56% inhibition at 50 mM Aureobasidium pullulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 3.7
-
p-nitrophenyl-alpha-L-arabinofuranoside
-
Aureobasidium pullulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 36000
-
gel filtration Aureobasidium pullulans
3.2.1.55 49000
-
1 * 49000, SDS-PAGE Aureobasidium pullulans
3.2.1.55 49900
-
1 * 49900, calculated from sequence Aureobasidium pullulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Aureobasidium pullulans Q6RKQ1
-
-
3.2.1.55 Aureobasidium pullulans NRRL Y-2311-1 Q6RKQ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 recombinant enzyme Aureobasidium pullulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 arabinoxylan + H2O the enzyme releases arabinose from maize fiber, oat spelt arabinoxylan, and wheat arabinoxylan, but not from larch wood arabinogalactan or alpha-1,5-debranched arabinan Aureobasidium pullulans ?
-
?
3.2.1.55 arabinoxylan + H2O the enzyme releases arabinose from maize fiber, oat spelt arabinoxylan, and wheat arabinoxylan, but not from larch wood arabinogalactan or alpha-1,5-debranched arabinan Aureobasidium pullulans NRRL Y-2311-1 ?
-
?
3.2.1.55 alpha-1,5-L-arabinopentaose + H2O low activity Aureobasidium pullulans L-arabinose + ?
-
?
3.2.1.55 alpha-1,5-L-arabinopentaose + H2O low activity Aureobasidium pullulans NRRL Y-2311-1 L-arabinose + ?
-
?
3.2.1.55 alpha-1,5-L-arabinotetraose + H2O low activity Aureobasidium pullulans L-arabinose + ?
-
?
3.2.1.55 alpha-1,5-L-arabinotetraose + H2O low activity Aureobasidium pullulans NRRL Y-2311-1 L-arabinose + ?
-
?
3.2.1.55 alpha-1,5-L-arabinotriose + H2O low activity Aureobasidium pullulans L-arabinose + ?
-
?
3.2.1.55 alpha-1,5-L-arabinotriose + H2O low activity Aureobasidium pullulans NRRL Y-2311-1 L-arabinose + ?
-
?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Aureobasidium pullulans p-nitrophenol + L-arabinofuranose
-
?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
Aureobasidium pullulans NRRL Y-2311-1 p-nitrophenol + L-arabinofuranose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 monomer 1 * 49000, SDS-PAGE Aureobasidium pullulans
3.2.1.55 monomer 1 * 49900, calculated from sequence Aureobasidium pullulans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 AkabfA
-
Aureobasidium pullulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 55
-
-
Aureobasidium pullulans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.55 45 60 45°C: about 55% of maximal activity, 60°C: about 70% of maximal activity Aureobasidium pullulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 50
-
2 h, stable Aureobasidium pullulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 3.5 4
-
Aureobasidium pullulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 2 5 pH 2.0: about 65% of maximal activity, pH 5.0: about 50% of maximal activity Aureobasidium pullulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.55 6
-
15 min, complete inactivation Aureobasidium pullulans
3.2.1.55 3
-
half-life: 29 min Aureobasidium pullulans
3.2.1.55 4 5 2 h, stable Aureobasidium pullulans
3.2.1.55 2.2
-
15 min, complete inactivation Aureobasidium pullulans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.55 Aureobasidium pullulans calculated from sequence
-
4.97