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Literature summary extracted from

  • Canakci, S.; Belduz, A.O.; Saha, B.C.; Yasar, A.; Ayaz, F.A.; Yayli, N.
    Purification and characterization of a highly thermostable alpha-L-arabinofuranosidase from Geobacillus caldoxylolyticus TK4 (2007), Appl. Microbiol. Biotechnol., 75, 813-820.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 a histidine tag is introduced at the N-terminal end of AbfATK4, and the recombinant protein is expressed in Escherichia coli BL21, under control of isopropyl-beta-D-thiogalactopyranoside-inducible T7 promoter Parageobacillus caldoxylosilyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 Cu2+ 1 mM, complete inhibition Parageobacillus caldoxylosilyticus
3.2.1.55 Hg2+ 1 mM, complete inhibition Parageobacillus caldoxylosilyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.17
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Parageobacillus caldoxylosilyticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.55 extracellular
-
 Parageobacillus caldoxylosilyticus
-
-
3.2.1.55 intracellular
-
 Parageobacillus caldoxylosilyticus 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.55 Co2+ stimulates Parageobacillus caldoxylosilyticus
3.2.1.55 Mn2+ stimulates Parageobacillus caldoxylosilyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 59000
-
 4 * 59000, SDS-PAGE Parageobacillus caldoxylosilyticus
3.2.1.55 236000
-
 gel filtration Parageobacillus caldoxylosilyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Parageobacillus caldoxylosilyticus
-
-
-
3.2.1.55 Parageobacillus caldoxylosilyticus TK4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55
-
 Parageobacillus caldoxylosilyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 additional information no endoarabinanase activity Parageobacillus caldoxylosilyticus ?
-
 ?
3.2.1.55 additional information no endoarabinanase activity Parageobacillus caldoxylosilyticus TK4 ?
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Parageobacillus caldoxylosilyticus p-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Parageobacillus caldoxylosilyticus TK4 p-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 p-nitrophenyl-beta-D-xylopyranoside + H2O low activity Parageobacillus caldoxylosilyticus p-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.55 p-nitrophenyl-beta-D-xylopyranoside + H2O low activity Parageobacillus caldoxylosilyticus TK4 p-nitrophenol + beta-D-xylopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 tetramer 4 * 59000, SDS-PAGE Parageobacillus caldoxylosilyticus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 AbfATK4
-
 Parageobacillus caldoxylosilyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 75 80
-
 Parageobacillus caldoxylosilyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 60 65 96 h, stable Parageobacillus caldoxylosilyticus
3.2.1.55 70
-
 48 h, stable Parageobacillus caldoxylosilyticus
3.2.1.55 75
-
 12 h, stable Parageobacillus caldoxylosilyticus
3.2.1.55 80
-
 30 min, 10% loss of activity. Complete loss of activity after 80 min Parageobacillus caldoxylosilyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 6
-
-
 Parageobacillus caldoxylosilyticus