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Literature summary extracted from

  • Dong, J.; Tamaru, Y.; Araki, T.
    A unique beta-agarase, AgaA, from a marine bacterium, Vibrio sp. strain PO-303 (2007), Appl. Microbiol. Biotechnol., 74, 1248-1255.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.81 the deduced enzyme protein contains a N-terminal signal peptide of 29 amino acid residues, followed by a 266 amino acid sequence that is homologous to catalytic module of family 16 glycoside hydrolases, a bacterial immunoglobulin group 2-like domain of 52 amino acid residues, two carbohydratebinding modules of family 6 separated from Big-2-like domain by nine times repeated GDDTDP amino acid sequence. Expression in Escherichia coli Vibrio sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.81 AlCl3 complete inhibition Vibrio sp.
3.2.1.81 CuCl2 complete inhibition Vibrio sp.
3.2.1.81 CuSO4 complete inhibition Vibrio sp.
3.2.1.81 HgCl2 complete inhibition Vibrio sp.
3.2.1.81 Pb(CH3COO)2 complete inhibition Vibrio sp.
3.2.1.81 sodium dodecylsulfate 0.1%, complete inhibition Vibrio sp.
3.2.1.81 ZnCl2 complete inhibition Vibrio sp.
3.2.1.81 ZnSO4 complete inhibition Vibrio sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.81 additional information
-
 additional information Km value for agarose is 1.1 mg/ml Vibrio sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.81 CaCl2 slight activation Vibrio sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.81 106062
-
 x * 106062, calculated, x * 120000, SDS-PAGE of recombinant protein Vibrio sp.
3.2.1.81 120000
-
 x * 106062, calculated, x * 120000, SDS-PAGE of recombinant protein Vibrio sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.81 Vibrio sp. Q0KKW9
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.81 proteolytic modification sequence contains a signal peptide of 29 amino acids Vibrio sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.81 16.4
-
 pH 7.5, 40°C Vibrio sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.81 agar + H2O
-
 Vibrio sp. neoagarohexaose + neoagarotetraose
-
 ?
3.2.1.81 agarose + H2O
-
 Vibrio sp. neoagarohexaose + neoagarotetraose
-
 ?
3.2.1.81 additional information no substrate: neoagarotetraose, neoagarobiose, kappa-carrageenan Vibrio sp. ?
-
 ?
3.2.1.81 neoagarohexaose + H2O poor substrate Vibrio sp. ?
-
 ?
3.2.1.81 neoagarooctaose + H2O
-
 Vibrio sp. neoagarohexaose + neoagarotetraose + neoagarobiose
-
 ?
3.2.1.81 porphyran + H2O
-
 Vibrio sp. neoagarohexaose + neoagarotetraose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.81 ? x * 106062, calculated, x * 120000, SDS-PAGE of recombinant protein Vibrio sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.81 beta-agarase-a
-
 Vibrio sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.81 40
-
-
 Vibrio sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.81 37
-
 stable below Vibrio sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.81 7.5
-
-
 Vibrio sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.81 5.5 11 stable at 25°C for 24 h Vibrio sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.81 Vibrio sp. calculated
-
 4.1