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Literature summary extracted from

  • Berrin, J.G.; Ajandouz, e.l.H.; Georis, J.; Arnaut, F.; Juge, N.
    Substrate and product hydrolysis specificity in family 11 glycoside hydrolases: an analysis of Penicillium funiculosum and Penicillium griseofulvum xylanases (2007), Appl. Microbiol. Biotechnol., 74, 1001-1010.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 expressed in Escherichia coli BL21 (pLys) cells as glutathione S-transferase fusion protein Penicillium griseofulvum
3.2.1.8 expressed in Escherichia coli BL21 (pLys) cells as glutathione S-transferase fusion protein Talaromyces funiculosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 additional information XynA is insensitive towards protein XIP-I Penicillium griseofulvum
3.2.1.8 protein XIP-I
-
 Talaromyces funiculosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 20800
-
 SDS-PAGE Penicillium griseofulvum
3.2.1.8 20830
-
 MALDI-TOF mass spectrometry Penicillium griseofulvum
3.2.1.8 21140
-
 MALDI-TOF mass spectrometry Talaromyces funiculosus
3.2.1.8 23200
-
 SDS-PAGE Talaromyces funiculosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Penicillium griseofulvum
-
-
-
3.2.1.8 Talaromyces funiculosus Q9HFH0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant enzyme is purified via glutathione-agarose beads, native enzyme is purified via Sephacryl S-200 gel filtration Penicillium griseofulvum
3.2.1.8 recombinant enzyme is purified via glutathione-agarose beads, native enzyme is purified via Sephacryl S-200 gel filtration Talaromyces funiculosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birchwood xylan + H2O
-
 Penicillium griseofulvum ?
-
 ?
3.2.1.8 birchwood xylan + H2O
-
 Talaromyces funiculosus ?
-
 ?
3.2.1.8 low-viscosity wheat arabinoxylan + H2O
-
 Penicillium griseofulvum ?
-
 ?
3.2.1.8 low-viscosity wheat arabinoxylan + H2O
-
 Talaromyces funiculosus ?
-
 ?
3.2.1.8 medium viscosity wheat arabinoxylan + H2O
-
 Penicillium griseofulvum ?
-
 ?
3.2.1.8 medium viscosity wheat arabinoxylan + H2O
-
 Talaromyces funiculosus ?
-
 ?
3.2.1.8 additional information no detectable activity on xylobiose Penicillium griseofulvum ?
-
 ?
3.2.1.8 additional information no detectable activity on xylobiose Talaromyces funiculosus ?
-
 ?
3.2.1.8 wheat soluble arabinoxylan + H2O
-
 Penicillium griseofulvum xylotriose + xylobiose predominant production of xylotriose and xylobiose as end products ?
3.2.1.8 wheat soluble arabinoxylan + H2O
-
 Talaromyces funiculosus xylotriose + xylobiose predominant production of xylotriose and xylobiose as end products ?
3.2.1.8 xylohexaose + H2O rapid cleavage Penicillium griseofulvum 2 xylotriose xylohexaose is cleaved initially to produce mainly xylotriose and a small amount of xylobiose and xylotetraose ?
3.2.1.8 xylohexaose + H2O rapid cleavage Talaromyces funiculosus 2 xylotriose xylohexaose is cleaved initially to produce mainly xylotriose and a small amount of xylobiose and xylotetraose ?
3.2.1.8 xylopentaose + H2O rapid cleavage Penicillium griseofulvum xylobiose + xylotriose
-
 ?
3.2.1.8 xylopentaose + H2O rapid cleavage Talaromyces funiculosus xylobiose + xylotriose
-
 ?
3.2.1.8 xylotetraose + H2O low activity Penicillium griseofulvum 2 xylobiose
-
 ?
3.2.1.8 xylotetraose + H2O low activity Talaromyces funiculosus 2 xylobiose
-
 ?
3.2.1.8 xylotriose + H2O low activity Penicillium griseofulvum ?
-
 ?
3.2.1.8 xylotriose + H2O low activity Talaromyces funiculosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase
-
 Penicillium griseofulvum
3.2.1.8 xylanase
-
 Talaromyces funiculosus
3.2.1.8 XynA
-
 Penicillium griseofulvum
3.2.1.8 XynC
-
 Talaromyces funiculosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 50 60
-
 Penicillium griseofulvum
3.2.1.8 50 60
-
 Talaromyces funiculosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 30
-
 stable at 30°C for 60 min, with more than 95% of the activity remaining Penicillium griseofulvum
3.2.1.8 30
-
 stable at 30°C for 60 min, with more than 95% of the activity remaining Talaromyces funiculosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 277
-
 birchwood xylan apparent value for the native enzyme Penicillium griseofulvum
3.2.1.8 292
-
 birchwood xylan apparent value for the recombinant enzyme Penicillium griseofulvum
3.2.1.8 872
-
 birchwood xylan apparent value for the native enzyme Talaromyces funiculosus
3.2.1.8 997
-
 birchwood xylan apparent value for the recombinant enzyme Talaromyces funiculosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5.5
-
-
 Penicillium griseofulvum
3.2.1.8 5.5
-
-
 Talaromyces funiculosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 4.5 6.5 displays over 50% activity in the pH range of 4.5 to 6.5 Penicillium griseofulvum
3.2.1.8 4.5 6.5 displays over 50% activity in the pH range of 4.5 to 6.5 Talaromyces funiculosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 7.5
-
 around 30% activity is still observed at pH 7.5 Penicillium griseofulvum
3.2.1.8 7.5
-
 no activity is observed at pH 7.5 Talaromyces funiculosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Penicillium griseofulvum isoelectric focusing
-
 4.2
3.2.1.8 Talaromyces funiculosus isoelectric focusing
-
 8.6