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Literature summary extracted from

  • Arai, T.; Kosugi, A.; Chan, H.; Koukiekolo, R.; Yukawa, H.; Inui, M.; Doi, R.H.
    Properties of cellulosomal family 9 cellulases from Clostridium cellulovorans (2006), Appl. Microbiol. Biotechnol., 71, 654-660.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4
-
 Clostridium cellulovorans

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.4 additional information EngHDELTACBM devoid of the carbohydrate binding module loses activity toward all substrates including carboxymethylcellulose Clostridium cellulovorans
3.2.1.4 additional information EngMDELTSACBM, devoid of the carbohydrate binding module has activity toward CMC (0.003 U/mg), in contrast with no activity toward any other substrate Clostridium cellulovorans
3.2.1.4 additional information EngYDELTSACBM, devoid of the carbohydrate binding module loses activity toward all substrates including carboxymethylcellulose Clostridium cellulovorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Clostridium cellulovorans O65987
-
-
3.2.1.4 Clostridium cellulovorans Q9AQF4
-
-
3.2.1.4 Clostridium cellulovorans Q9LAJ2
-
-
3.2.1.4 Clostridium cellulovorans Q9RGE6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Clostridium cellulovorans
3.2.1.4 EngH and truncated derivative Clostridium cellulovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 acid-swollen cellulose + H2O
-
 Clostridium cellulovorans ?
-
 ?
3.2.1.4 acid-swollen cellulose + H2O cleaved cellulose randomly Clostridium cellulovorans ?
-
 ?
3.2.1.4 avicel + H2O
-
 Clostridium cellulovorans ?
-
 ?
3.2.1.4 beta-glucan + H2O
-
 Clostridium cellulovorans ?
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Clostridium cellulovorans ?
-
 ?
3.2.1.4 cellohexaose + H2O
-
 Clostridium cellulovorans ?
-
 ?
3.2.1.4 cellooligosaccharides + H2O random cleavage, slow reaction rates for cellooligosaccharides smaller than G5 Clostridium cellulovorans ?
-
 ?
3.2.1.4 cellopentaose + H2O
-
 Clostridium cellulovorans ?
-
 ?
3.2.1.4 cellotetraose + H2O
-
 Clostridium cellulovorans ?
-
 ?
3.2.1.4 lichenan + H2O
-
 Clostridium cellulovorans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 endoglucanase H
-
 Clostridium cellulovorans
3.2.1.4 endoglucanase L
-
 Clostridium cellulovorans
3.2.1.4 endoglucanase M
-
 Clostridium cellulovorans
3.2.1.4 endoglucanase Y
-
 Clostridium cellulovorans
3.2.1.4 EngH
-
 Clostridium cellulovorans
3.2.1.4 EngL
-
 Clostridium cellulovorans
3.2.1.4 EngM
-
 Clostridium cellulovorans
3.2.1.4 EngY
-
 Clostridium cellulovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 37
-
-
 Clostridium cellulovorans
3.2.1.4 42
-
-
 Clostridium cellulovorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 40
-
 stable below Clostridium cellulovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6
-
-
 Clostridium cellulovorans
3.2.1.4 6.5
-
-
 Clostridium cellulovorans
3.2.1.4 7
-
-
 Clostridium cellulovorans
3.2.1.4 8
-
-
 Clostridium cellulovorans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 5 9 stable Clostridium cellulovorans
3.2.1.4 5 8 stable Clostridium cellulovorans