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Literature summary extracted from

  • Salameh, M.A.; Wiegel, J.
    Purification and characterization of two highly thermophilic alkaline lipases from Thermosyntropha lipolytica (2007), Appl. Environ. Microbiol., 73, 7725-7731.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.1.1.3 2 mM ammonium sulfate, bovine serum albumin at 2 mg/ml, or 40% v/v glycerol stabilize the cold-labile purified enzyme Thermosyntropha lipolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Ca2+ the enzyme activity is not affected by Ca2+ at 1-10 mM, 15% inhibition at 25 mM at pH 9.4-9.6 Thermosyntropha lipolytica
3.1.1.3 EDTA complete inhibition at 10 mM Thermosyntropha lipolytica
3.1.1.3 PMSF complete inhibition at 10 mM Thermosyntropha lipolytica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular LipA and LipB Thermosyntropha lipolytica
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ both LipA and LipB contain one ion per monomeric enzyme unit, the enzyme activity is not affected by Ca2+ at 1-10 mM, 15% inhibition at 25 mM at pH 9.4-9.6 Thermosyntropha lipolytica
3.1.1.3 Mn2+ both LipA and LipB contain one ion per monomeric enzyme unit, the addition of 1 mM MnCl2 to dialyzed enzyme preparations enhances the activities at 96°C of both LipA and LipB by 3fold and increases the durations of their thermal stability at 60°C and 75°C, respectively, by 4 h Thermosyntropha lipolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 50000
-
 x * 50000, LipA, SDS-PAGE, x * 57000, LipB, SDS-PAGE Thermosyntropha lipolytica
3.1.1.3 57000
-
 x * 50000, LipA, SDS-PAGE, x * 57000, LipB, SDS-PAGE Thermosyntropha lipolytica

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Thermosyntropha lipolytica
-
 strain DSM 11003, an anaerobic, thermophilic, alkali-tolerant bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 native LipA and LipB 140fold and 134fold, respectively, from cell culture supernatant by ammonium sulfate fractionation amd hydrophobic interaction chromatography to homogeneity Thermosyntropha lipolytica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 cell culture optimal growth at pH 7.6 and 60°C, overview Thermosyntropha lipolytica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 13.4
-
 purified LipB Thermosyntropha lipolytica
3.1.1.3 14
-
 purified LipA Thermosyntropha lipolytica

Storage Stability

EC Number Storage Stability Organism
3.1.1.3 0-4°C, purified LipA and LipB, irreversible loss of activity of both isozymes, after 24 h or when frozen in various buffers, including phosphate buffer, pH 8.0, determined at 25°C, bovine serum albumin at 2 mg/ml or 40% v/v glycerol stabilize the purified enzyme Thermosyntropha lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Thermosyntropha lipolytica 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Thermosyntropha lipolytica 4-nitrophenol + palmitate
-
 ?
3.1.1.3 additional information LipA and LipB exhibit high activity with long-chain fatty acid glycerides, yielding maximum activity with trioleate and, among the 4-nitrophenyl esters, with 4-nitrophenyl laurate, hydrolysis of glycerol ester bonds occurs at positions 1 and 3, tributyrin and tricaproin are poor substrates, overview Thermosyntropha lipolytica ?
-
 ?
3.1.1.3 triolein + H2O
-
 Thermosyntropha lipolytica diolein + oleate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 50000, LipA, SDS-PAGE, x * 57000, LipB, SDS-PAGE Thermosyntropha lipolytica

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 LipA
-
 Thermosyntropha lipolytica
3.1.1.3 LipB
-
 Thermosyntropha lipolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 96
-
 about, LipA and LipB Thermosyntropha lipolytica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 additional information
-
 addition of 1 mM MnCl2 to dialyzed enzyme preparations increases the durations of their thermal stability at 60°C and 75°C, respectively, by 4 h Thermosyntropha lipolytica
3.1.1.3 100
-
 LipA and LipB, at pH 8.0, determined at 25°C, retain 50% activity after 6 h and 2 h of incubation in presence of 2 M ammonium sulfate, respectively Thermosyntropha lipolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8.2
-
 assay at, pH is determined at 25°C Thermosyntropha lipolytica