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Literature summary extracted from

  • Seo, J.M.; Ji, G.E.; Cho, S.H.; Park, M.S.; Lee, H.J.
    Characterization of a Bifidobacterium longum BORI dipeptidase belonging to the U34 family (2007), Appl. Environ. Microbiol., 73, 5598-5606.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.13.19 expressed in Escherichia coli Bifidobacterium longum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.13.19 49000
-
 native enzyme, SDS-PAGE Bifidobacterium longum

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.19 Bifidobacterium longum
-
-
-
3.4.13.19 Bifidobacterium longum BORI
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.19 recombinant enzyme is purified by nickel-chelating affinity chromatography, wild type enzyme is purified by ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Q-Sepharose column chromatography Bifidobacterium longum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.13.19 additional information
-
 0.71 units/mg from cell extract, 77.2 units/mg after 109fold purification, one unit is defined as that required to increase the absorbance by one unit per minute Bifidobacterium longum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.19 Ala-Ala + H2O 62.5% activity compared to Ala-Gln Bifidobacterium longum Ala + Ala
-
 ?
3.4.13.19 Ala-Ala + H2O 62.5% activity compared to Ala-Gln Bifidobacterium longum BORI Ala + Ala
-
 ?
3.4.13.19 Ala-Gln + H2O 100% activity Bifidobacterium longum Ala + Gln
-
 ?
3.4.13.19 Ala-Gly + H2O 68.9% activity compared to Ala-Gln Bifidobacterium longum Ala + Gly
-
 ?
3.4.13.19 Ala-Gly + H2O 68.9% activity compared to Ala-Gln Bifidobacterium longum BORI Ala + Gly
-
 ?
3.4.13.19 Ala-Leu + H2O 14.0% activity compared to Ala-Gln Bifidobacterium longum Ala + Leu
-
 ?
3.4.13.19 Ala-Phe + H2O 14.0% activity compared to Ala-Gln Bifidobacterium longum Ala + Phe
-
 ?
3.4.13.19 Ala-Phe + H2O 14.0% activity compared to Ala-Gln Bifidobacterium longum BORI Ala + Phe
-
 ?
3.4.13.19 Arg-Phe + H2O 6.3% activity compared to Ala-Gln Bifidobacterium longum Arg + Phe
-
 ?
3.4.13.19 Asp-Phe + H2O 4.9% activity compared to Ala-Gln Bifidobacterium longum Asp + Phe
-
 ?
3.4.13.19 Leu-Leu + H2O 71% activity compared to Ala-Gln Bifidobacterium longum Leu + Leu
-
 ?
3.4.13.19 Leu-Phe + H2O 12.5% activity compared to Ala-Gln Bifidobacterium longum Leu + Phe
-
 ?
3.4.13.19 Leu-Trp + H2O 10.2% activity compared to Ala-Gln Bifidobacterium longum Leu + Trp
-
 ?
3.4.13.19 additional information low activity toward dipeptides containing aromatic amino acids except for Phe-Gly, does not hydrolyze Leu-Asp, Phe-Phe, or N- or C-terminus-blocked dipeptides (N-carbobenzyloxy-Phe-Gly, N-carbobenzyloxy-Ala-Leu, or Leu-Leu-amide), does not hydrolyze tri-, tetra-, or pentapeptides or p-nitroanilide derivatives of dipeptides and peptides Bifidobacterium longum ?
-
 ?
3.4.13.19 additional information low activity toward dipeptides containing aromatic amino acids except for Phe-Gly, does not hydrolyze Leu-Asp, Phe-Phe, or N- or C-terminus-blocked dipeptides (N-carbobenzyloxy-Phe-Gly, N-carbobenzyloxy-Ala-Leu, or Leu-Leu-amide), does not hydrolyze tri-, tetra-, or pentapeptides or p-nitroanilide derivatives of dipeptides and peptides Bifidobacterium longum BORI ?
-
 ?
3.4.13.19 Phe-Ala + H2O 6.7% activity compared to Ala-Gln Bifidobacterium longum Phe + Ala
-
 ?
3.4.13.19 Phe-Gly + H2O 74.1% activity compared to Ala-Gln Bifidobacterium longum Phe + Gly
-
 ?
3.4.13.19 Phe-Gly + H2O 74.1% activity compared to Ala-Gln Bifidobacterium longum BORI Phe + Gly
-
 ?
3.4.13.19 Tyr-Leu + H2O 9.5% activity compared to Ala-Gln Bifidobacterium longum Tyr + Leu
-
 ?
3.4.13.19 Tyr-Phe + H2O 3.8% activity compared to Ala-Gln Bifidobacterium longum Tyr + Phe
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.13.19 dipeptidase capable of hydrolyzing a range of dipeptides but not tri- and tetrapeptides, p-nitroanilide derivatives of amino acids, or N- or C-terminus-blocked dipeptides Bifidobacterium longum
3.4.13.19 PepD
-
 Bifidobacterium longum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.13.19 50
-
-
 Bifidobacterium longum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.13.19 40 60
-
 Bifidobacterium longum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.13.19 20 70 the enzyme shows about 30% and 50% of the maximum activity at 20°C and 70°C, respectively, stable at temperatures up to 70°C, but the activity decreased rapidly above 80°C Bifidobacterium longum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.19 8
-
-
 Bifidobacterium longum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.13.19 7 9
-
 Bifidobacterium longum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.13.19 4 10 very low activity (less than about 10%) is observed below pH 5.5, at pH 10.0 the activity is about 50% of the maximum, the enzyme is stable for 30 min over the pH range 4.0 to 8.0 Bifidobacterium longum