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Literature summary extracted from
- Heterologous expression and enzymatic characterization of beta-glucosidase from the drywood-eating termite, Neotermes koshunensis (2007), Appl. Entomol. Zool., 42, 457-463.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | overexpression of His-tagged enzyme in Escherichia coli strain JM109 | Neotermes koshunensis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 3.8 | - |
cellobiose | pH 5.5, 37°C, recombinant His-tagged enzyme | Neotermes koshunensis |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 60000 | - |
x * 60000, recombinant His-tagged enzyme, SDS-PAGE | Neotermes koshunensis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | cellobiose + H2O | Neotermes koshunensis | - |
2 beta-D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Neotermes koshunensis | Q8T0W7 | drywood-eating termite | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | recombinant His-tagged enzyme 6.4fold from Escherichia coli to homogeneity by nickel affinity chromatography | Neotermes koshunensis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 156.7 | - |
purified recombinant His-tagged enzyme | Neotermes koshunensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-nitrophenyl beta-D-fucopyranoside + H2O | high activity | Neotermes koshunensis | 4-nitrophenol + beta-D-fucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-galactopyranoside + H2O | low activity | Neotermes koshunensis | 4-nitrophenol + beta-D-galactose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | low activity | Neotermes koshunensis | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Neotermes koshunensis | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | high activity | Neotermes koshunensis | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | cellotetraose + 3 H2O | low activity | Neotermes koshunensis | 4 beta-D-glucose | - |
? | |
| 3.2.1.21 | cellotriose + H2O | low activity | Neotermes koshunensis | 3 D-glucose | - |
? | |
| 3.2.1.21 | laminaribiose + H2O | high activity | Neotermes koshunensis | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | laminarin + H2O | low activity | Neotermes koshunensis | beta-D-glucose | - |
? | |
| 3.2.1.21 | additional information | substrate specificity of the recombinant His-tagged enzyme, gentiobiose, lactose, and salicin are poor substrates, melibiose, palatinose, sophorose, and cellulose are no substrates, overview | Neotermes koshunensis | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | ? | x * 60000, recombinant His-tagged enzyme, SDS-PAGE | Neotermes koshunensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50 | - |
recombinant His-tagged enzyme | Neotermes koshunensis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 10 | 50 | activity range | Neotermes koshunensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 40 | - |
purified recombinant His-tagged enzyme, 30 min, 80% remianing activity | Neotermes koshunensis |
| 3.2.1.21 | 45 | - |
purified recombinant His-tagged enzyme, stable up to | Neotermes koshunensis |
| 3.2.1.21 | 50 | - |
purified recombinant His-tagged enzyme, inactivation | Neotermes koshunensis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5 | - |
recombinant His-tagged enzyme | Neotermes koshunensis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4 | 8.5 | activity range | Neotermes koshunensis |
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