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Literature summary extracted from

  • Gibson, R.P.; Gloster, T.M.; Roberts, S.; Warren, R.A.; Storch de Gracia, I.; Garcia, A.; Chiara, J.L.; Davies, G.J.
    Molecular basis for trehalase inhibition revealed by the structure of trehalase in complex with potent inhibitors (2007), Angew. Chem., 46, 4115-4119.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.28 expressed in Escherichia coli BL21 (DE3) cells Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 1-thiatrehazolin
-
 Escherichia coli
3.2.1.28 validoxylamine A
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 0.41
-
 trehalose at 37°C in sodium maleate buffer (75 mM, pH 5.5) Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Escherichia coli P13482
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28 with PEG 3350 (25% w/v) and bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane (Bis-Tris)/HCl buffer (0.1 m, pH 6.5) Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 trehalose + H2O
-
 Escherichia coli D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.28 GH37 trehalase
-
 Escherichia coli
3.2.1.28 Tre37A
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.28 199
-
 trehalose at 37°C in sodium maleate buffer (75 mM, pH 5.5) Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.28 0.000009
-
 1-thiatrehazolin at 37°C in sodium maleate buffer (75 mM, pH 5.5) Escherichia coli
3.2.1.28 0.00001
-
 validoxylamine A at 37°C in sodium maleate buffer (75 mM, pH 5.5) Escherichia coli