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Literature summary extracted from

  • Iqbal, J.; Jirovsky, D.; Lee, S.Y.; Zimmermann, H.; Mueller, C.E.
    Capillary electrophoresis-based nanoscale assays for monitoring ecto-5'-nucleotidase activity and inhibition in preparations of recombinant enzyme and melanoma cell membranes (2008), Anal. Biochem., 373, 129-140.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.5 catalytically active recombinant soluble glutathione-S transferase/ecto-5'-NT fusion protein is expressed in insect cells using the baculovirus system Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 ADP
-
 Homo sapiens
3.1.3.5 ADP
-
 Rattus norvegicus
3.1.3.5 alpha,beta-methylene-ADP
-
 Homo sapiens
3.1.3.5 alpha,beta-methylene-ADP
-
 Rattus norvegicus
3.1.3.5 ATP
-
 Homo sapiens
3.1.3.5 ATP
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 membrane
-
 Homo sapiens 16020
-
3.1.3.5 membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Mg2+ dependent Homo sapiens
3.1.3.5 Mg2+ dependent Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Homo sapiens
-
-
-
3.1.3.5 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5 agarose-coupled GSH affinity chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 melanoma cell
-
 Homo sapiens
-
3.1.3.5 melanoma cell
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Homo sapiens adenosine + phosphate
-
 ?
3.1.3.5 5'-AMP + H2O 100% activity Rattus norvegicus adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O 36% dephosphorylation in relation to AMP Rattus norvegicus cytidine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O 57% dephosphorylation in relation to AMP Rattus norvegicus guanosine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O 51% dephosphorylation in relation to AMP Rattus norvegicus uridine + phosphate
-
 ?
3.1.3.5 p-nitrophenyl phosphate + H2O
-
 Homo sapiens p-nitrophenol + phosphate
-
 ?
3.1.3.5 p-nitrophenyl phosphate + H2O
-
 Rattus norvegicus p-nitrophenol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.5 CD73
-
 Homo sapiens
3.1.3.5 CD73
-
 Rattus norvegicus
3.1.3.5 ecto-5'-NT
-
 Homo sapiens
3.1.3.5 ecto-5'-NT
-
 Rattus norvegicus
3.1.3.5 ecto-5'-nucleotidase
-
 Homo sapiens
3.1.3.5 ecto-5'-nucleotidase
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.5 0.00051
-
 alpha,beta-methylene-ADP in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2 Homo sapiens
3.1.3.5 0.00087
-
 alpha,beta-methylene-ADP in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2 Rattus norvegicus
3.1.3.5 0.00091
-
 ADP in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2 Rattus norvegicus
3.1.3.5 0.0089
-
 ATP in 10 mM HEPES (pH 7.4), 2 mM MgCl2, and 1 mM CaCl2 Rattus norvegicus