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Literature summary extracted from
- Enzymatic assay of D-serine using D-serine dehydratase from Saccharomyces cerevisiae (2007), Anal. Biochem., 371, 167-172.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.3.1.18 | medicine | the established enzymatic method could be used for the quantitative determination of D-serine in human urine | Saccharomyces cerevisiae |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.3.1.18 | into the pET15b vector for expression in Escherichia coli BL21DE3 cells | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.1.18 | D-serine | Saccharomyces cerevisiae | - |
pyruvate + NH3 | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.1.18 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.1.18 | using a Ni-chelating DEAE-TOYOPEARL and a gel permeation column, from 1 l culture 8 mg Dsd1p is obtained | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.1.18 | D-serine | - |
Saccharomyces cerevisiae | pyruvate + NH3 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.3.1.18 | D-serine ammonia-lyase | - |
Saccharomyces cerevisiae |
| 4.3.1.18 | D-serine dehydratase | - |
Saccharomyces cerevisiae |
| 4.3.1.18 | DSD | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.18 | 30 | - |
activity assayed by the determination of the ammonia formend from D-serine | Saccharomyces cerevisiae |
| 4.3.1.18 | 37 | - |
D-serine assay with the LDH-coupling method, the amount of released pyruvate is measured | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.18 | 8 | - |
activity assay | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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