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Literature summary extracted from

  • Jaenis, J.; Pulkkinen, P.; Rouvinen, J.; Vainiotalo, P.
    Determination of steady-state kinetic parameters for a xylanase-catalyzed hydrolysis of neutral underivatized xylooligosaccharides by mass spectrometry (2007), Anal. Biochem., 365, 165-173.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 0.073
-
 xylohexaose in 10 mM ammonium acetate buffer at pH 5.0 and 20°C Trichoderma reesei
3.2.1.8 0.136
-
 xylopentaose in 10 mM ammonium acetate buffer at pH 5.0 and 20°C Trichoderma reesei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 21000
-
 around 21000 Da, SDS-PAGE Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Trichoderma reesei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 additional information xylobiose is not hydrolyzed Trichoderma reesei ?
-
 ?
3.2.1.8 xylohexaose + H2O most preferred substrate of TRX II Trichoderma reesei xylotriose + xylotriose xylohexaose is hydrolyzed mainly to two xylotrioses, but considerable amounts of xylotetraose and xylobiose are also detected while only traceable amounts of xylopentaose are detected ?
3.2.1.8 xylopentaose + H2O
-
 Trichoderma reesei xylotriose + xylobiose xylopentaose is hydrolyzed mainly to xylotriose and xylobiose with only minor amounts of xylotetraose ?
3.2.1.8 xylotetraose + H2O
-
 Trichoderma reesei xylobiose + xylobiose xylotetraose is hydrolyzed mainly to two xylobioses, with only traceable amounts of xylotriose ?
3.2.1.8 xylotriose + H2O
-
 Trichoderma reesei xylobiose + xylose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 endo-1,4-beta-xylanase II
-
 Trichoderma reesei
3.2.1.8 TRX II
-
 Trichoderma reesei
3.2.1.8 xylanase
-
 Trichoderma reesei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 0.045
-
 xylotriose in 10 mM ammonium acetate buffer at pH 5.0 and 20°C Trichoderma reesei
3.2.1.8 0.162
-
 xylotetraose in 10 mM ammonium acetate buffer at pH 5.0 and 20°C Trichoderma reesei
3.2.1.8 50.3
-
 xylopentaose in 10 mM ammonium acetate buffer at pH 5.0 and 20°C Trichoderma reesei
3.2.1.8 68
-
 xylohexaose in 10 mM ammonium acetate buffer at pH 5.0 and 20°C Trichoderma reesei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Trichoderma reesei isoelectric focusing
-
 9