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Literature summary extracted from

  • Nilsson, C.; Nilsson, F.; Turner, P.; Sixtensson, M.; Nordberg Karlsson, E.; Holst, O.; Cohen, A.; Gorton, L.
    Characterisation of two novel cyclodextrinases using on-line microdialysis sampling with high-performance anion exchange chromatography (2006), Anal. Bioanal. Chem., 385, 1421-1429.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.54 expression in Escherichia coli Laceyella sacchari
3.2.1.54 expression in Escherichia coli Anoxybacillus flavithermus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Anoxybacillus flavithermus
-
-
-
3.2.1.54 Laceyella sacchari
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54 of the recombinant protein Laceyella sacchari
3.2.1.54 of the recombinant protein Anoxybacillus flavithermus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 alpha-cyclodextrin + H2O
-
 Laceyella sacchari maltose + glucose in an approximate molar ratio of 2 to 1, the intermediate products are analysed using a pulsed amperometric detector for on-line measurements and real-time sampling ?
3.2.1.54 alpha-cyclodextrin + H2O
-
 Anoxybacillus flavithermus maltose + glucose in an approximate molar ratio of 2 to 1, the intermediate products are analysed using a pulsed amperometric detector for on-line measurements and real-time sampling ?
3.2.1.54 beta-cyclodextrin + H2O
-
 Laceyella sacchari maltose + glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 beta-cyclodextrin + H2O
-
 Anoxybacillus flavithermus maltose + glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 cyclomaltodextrin + H2O
-
 Laceyella sacchari linear maltodextrin
-
 ?
3.2.1.54 cyclomaltodextrin + H2O
-
 Anoxybacillus flavithermus linear maltodextrin
-
 ?
3.2.1.54 gamma-cyclodextrin + H2O
-
 Laceyella sacchari maltose + glucose in an approximate molar ratio of 2 to 1 ?
3.2.1.54 gamma-cyclodextrin + H2O
-
 Anoxybacillus flavithermus maltose + glucose in an approximate molar ratio of 2 to 1 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.54 dimer
-
 Anoxybacillus flavithermus
3.2.1.54 monomer
-
 Laceyella sacchari

Synonyms

EC Number Synonyms Comment Organism
3.2.1.54 AfCda13
-
 Anoxybacillus flavithermus
3.2.1.54 CDase
-
 Laceyella sacchari
3.2.1.54 CDase
-
 Anoxybacillus flavithermus
3.2.1.54 cyclomaltodextrinase
-
 Laceyella sacchari
3.2.1.54 cyclomaltodextrinase
-
 Anoxybacillus flavithermus
3.2.1.54 LsCda13
-
 Laceyella sacchari

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.54 55
-
-
 Laceyella sacchari
3.2.1.54 55
-
-
 Anoxybacillus flavithermus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.54 7
-
-
 Laceyella sacchari
3.2.1.54 7
-
-
 Anoxybacillus flavithermus