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Literature summary extracted from

  • Pokharel, D.R.; Rathaur, S.
    Purification and characterization of a leucine aminopeptidase from the bovine filarial parasite Setaria cervi (2008), Acta Trop., 106, 1-8.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.11.1 medicine serodiagnostic marker against active filarial infection Setaria cervi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 1,10-phenanthroline 5.0 mM, 55% inhibition Setaria cervi
3.4.11.1 amastatin 0.01 mM, 81% inhibition Setaria cervi
3.4.11.1 Ba2+ 1 mM, 74% inhibition Setaria cervi
3.4.11.1 bestatin 0.001 mM, 58% inhibition Setaria cervi
3.4.11.1 Cd2+ 1 mM, 96% inhibition Setaria cervi
3.4.11.1 Cu2+ 1 mM, 75% inhibition Setaria cervi
3.4.11.1 EDTA 10 mM, 93% inhibition Setaria cervi
3.4.11.1 Fe2+ 1 mM, 73% inhibition Setaria cervi
3.4.11.1 glycine
-
 Setaria cervi
3.4.11.1 L-glutamine
-
 Setaria cervi
3.4.11.1 L-leucine
-
 Setaria cervi
3.4.11.1 Ni2+ 1 mM, 55% inhibition Setaria cervi
3.4.11.1 Pb2+ 1 mM, 60% inhibition Setaria cervi
3.4.11.1 Zn2+ 1 mM, 56% inhibition Setaria cervi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 8.8
-
 Leu-4-nitroanilide pH 8.0, 37°C Setaria cervi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Co2+ 1 mM, 5.3fold activation Setaria cervi
3.4.11.1 Mg2+ 1 mM, 4.7fold activation Setaria cervi
3.4.11.1 Mn2+ 1 mM, 3.9fold activation Setaria cervi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 54000
-
 6 * 54000, SDS-PAGE Setaria cervi
3.4.11.1 321000
-
 gel filtration Setaria cervi

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Setaria cervi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1
-
 Setaria cervi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 adult
-
 Setaria cervi
-
3.4.11.1 microfilarial stage
-
 Setaria cervi
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.1 18.2
-
-
 Setaria cervi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 Leu-4-nitroanilide + H2O
-
 Setaria cervi L-leucine + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 hexamer 6 * 54000, SDS-PAGE Setaria cervi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 37
-
-
 Setaria cervi

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.11.1 37 60 37°C: maximum, 60°C: 6.15% of maximal activity Setaria cervi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.1 70
-
 completely denatured at 70°C and above Setaria cervi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 9
-
-
 Setaria cervi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.1 8 9.5 pH 8.0: about 25% of maximal activity, pH 9.5: about 10% of maximal activity Setaria cervi