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Literature summary extracted from

  • Yoshida, A.; Tomita, T.; Kuzuyama, T.; Nishiyama, M.
    Purification, crystallization and preliminary X-ray analysis of the regulatory subunit of aspartate kinase from Thermus thermophilus (2007), Acta Crystallogr. Sect. F, 63, 96-98.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.4 into the NdeI/HindIII site of pET26b(+) and introduced into Escherichia coli BL21-CodonPlus(DE3)-RIL cells Thermus thermophilus
2.7.2.4 into vector pET26b(+) and introduced into Escherichia coli BL21-CodonPlus(DE3)-RIL cells Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.4 by hanging-drop vapour-diffusion method, regulatory subunit in the presence of the inhibitor threonine, to 2.15 A resolution, crystal belongs to the cubic space group P4332 or P4132, with unit-cell parameters a = b = c = 141.8 A Thermus thermophilus
2.7.2.4 by the hanging-drop vapour-diffusion method using Crystal Screen kits, the regulatory subunit (the beta subunit of Thermus thermophilus AK) is crystallized in the presence of the inhibitor threonine. Diffraction data are collected to 2.15 A at a synchrotron source. The crystal belongs to the cubic space group P4332 or P4132, with unit-cell parameters a = b = c = 141.8 A. Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.4 L-threonine feedback inhibition by the end product Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.4 17720
-
 full-length beta subunit (Met1Β–Ala161) with a His6-tag extension at the C-terminal end, calculated Thermus thermophilus
2.7.2.4 17720
-
 beta subunit (Met1-Ala161), calculated from nucleotide sequence Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.4 Thermus thermophilus P61489
-
-
2.7.2.4 Thermus thermophilus P61489 expression in Escherichia coli BL21-CodonPlus(DE3)-RIL cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.4 by nickel-affinity chromatography and gel filtration, to more than 95% homogeneity Thermus thermophilus
2.7.2.4 nickel-affinity chromatography, gel-filtration FPLC column, more than 95% homogeneity of the purified subunit is verified by SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.7.2.4 AK
-
 Thermus thermophilus
2.7.2.4 aspartate kinase
-
 Thermus thermophilus