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Literature summary extracted from

  • Hasan, F.; Shah, A.A.; Hameed, A.
    Purification and characterization of a mesophilic lipase from Bacillus subtilis FH5 stable at high temperature and pH (2007), Acta Biol. Hung., 58, 115-132.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 acetone inhibitory effect of organic solvents, overview Bacillus subtilis
3.1.1.3 Co2+
-
 Bacillus subtilis
3.1.1.3 Cu2+
-
 Bacillus subtilis
3.1.1.3 EDTA about 22% inhibition at 1 mM Bacillus subtilis
3.1.1.3 ethanol inhibitory effect of organic solvents, overview Bacillus subtilis
3.1.1.3 Isopropanol inhibitory effect of organic solvents, overview Bacillus subtilis
3.1.1.3 Na+
-
 Bacillus subtilis
3.1.1.3 PMSF about 15% inhibition at 1 mM Bacillus subtilis
3.1.1.3 SDS 20% inhibition at 1 mM Bacillus subtilis
3.1.1.3 Zn2+
-
 Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 5.05
-
 4-nitrophenyl laurate pH 8.0, 37°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ba2+ about 7.5% activation at 1 mM Bacillus subtilis
3.1.1.3 Ca2+ 35% activation at 1 mM Bacillus subtilis
3.1.1.3 Fe2+ 35% activation at 1 mM Bacillus subtilis
3.1.1.3 Mg2+ 40% activation at 1 mM Bacillus subtilis
3.1.1.3 Mn2+ 35% activation at 1 mM Bacillus subtilis
3.1.1.3 additional information the enzyme is not affected by 1 mM of K+ Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 24000
-
 x * 62000, SDS-PAGE x * 24000, SDS-PAGE Bacillus subtilis
3.1.1.3 62000
-
 x * 62000, SDS-PAGE x * 24000, SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Bacillus subtilis
-
 isolated from tannery wastes
-
3.1.1.3 Bacillus subtilis FH5
-
 isolated from tannery wastes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 native isozymes to homogeneity by acetone fractionation, anion exchange chromatography, gel filtration, and hydroxylapatite adsorption chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Bacillus subtilis 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Bacillus subtilis FH5 4-nitrophenol + laurate
-
 ?
3.1.1.3 long-chain triacylglycerol + H2O
-
 Bacillus subtilis diacylglycerol + a carboxylate
-
 ?
3.1.1.3 long-chain triacylglycerol + H2O
-
 Bacillus subtilis FH5 diacylglycerol + a carboxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 62000, SDS-PAGE x * 24000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 mesophilic lipase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 37
-
 assay at Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 40 90 temperature profile, overview Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 40
-
 1 h, purified native enzyme, 99% remaining activity Bacillus subtilis
3.1.1.3 50
-
 1 h, purified native enzyme, 92% remaining activity Bacillus subtilis
3.1.1.3 60
-
 1 h, purified native enzyme, 76% remaining activity Bacillus subtilis
3.1.1.3 70
-
 1 h, purified native enzyme, 38% remaining activity Bacillus subtilis
3.1.1.3 75
-
 1 h, purified native enzyme, 25% remaining activity Bacillus subtilis
3.1.1.3 85
-
 1 h, purified native enzyme, 9.7% remaining activity Bacillus subtilis
3.1.1.3 90
-
 1 h, purified native enzyme, 6.9% remaining activity Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 10
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 4 11 pH profile, overview Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 4
-
 1 h, 37°C, purified native enzyme, 14% remaining activity Bacillus subtilis
3.1.1.3 5
-
 1 h, 37°C, purified native enzyme, 24% remaining activity Bacillus subtilis
3.1.1.3 6
-
 1 h, 37°C, purified native enzyme, 44% remaining activity Bacillus subtilis
3.1.1.3 7
-
 1 h, 37°C, purified native enzyme, 52% remaining activity Bacillus subtilis
3.1.1.3 8
-
 1 h, 37°C, purified native enzyme, 68% remaining activity Bacillus subtilis
3.1.1.3 9
-
 1 h, 37°C, purified native enzyme, 74% remaining activity Bacillus subtilis
3.1.1.3 10
-
 1 h, 37°C, purified native enzyme, completely stable Bacillus subtilis
3.1.1.3 11
-
 1 h, 37°C, purified native enzyme, 90% remaining activity Bacillus subtilis