Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Swiegers, J.H.; Capone, D.L.; Pardon, K.H.; Elsey, G.M.; Sefton, M.A.; Francis, I.L.; Pretorius, I.S.
    Engineering volatile thiol release in Saccharomyces cerevisiae for improved wine aroma (2007), Yeast, 24, 561-574.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.99.1 food industry expression of enzyme in wine yeast results in a strong increase of passion fruit aroma in wine Escherichia coli
4.4.1.13 food industry transformed wine yeast strain has a 10fold higher activity of cysteine-S-conjugate beta-lyase Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.1 expressed in wine yeast strains Saccharomyces cerevisiae strains YHUM272a and VIN13, expression of enzyme results in a strong increase of passion fruit aroma in wine Escherichia coli
4.4.1.13 overexpression of cysteine-S-conjugate beta-lyase gene from Escherichia coli in a commercial wine yeast strain Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.1 L-Trp + H2O Escherichia coli
-
 indole + pyruvate + NH4+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.1 Escherichia coli
-
 tryptophanase with strong cysteine beta-lyase activity
-
4.4.1.13 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.1 L-Trp + H2O
-
 Escherichia coli indole + pyruvate + NH4+
-
 r
4.4.1.13 S-4-(4-methylpentan-2-one)-L-cysteine + H2O
-
 Escherichia coli 4-mercapto-4-methylpentan-2-one + NH3 + pyruvate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.13 cysteine-S-conjugate beta-lyase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.1 pyridoxal 5'-phosphate
-
 Escherichia coli