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Literature summary extracted from

  • Bougie, I.; Bisaillon, M.
    Characterization of the RNA binding energetics of the Candida albicans poly(A) polymerase (2007), Yeast, 24, 431-446.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.19 expressed in Escherichia coli BL21(DE3) cells Candida albicans

General Stability

EC Number General Stability Organism
2.7.7.19 the protein undergoes structural modifications upon RNA binding, although the interaction does not significantly modify the stability of the protein Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.19 ATP + RNA Candida albicans
-
 diphosphate + RNA(A)n
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.19 Candida albicans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.19 Ni-NTA agarose column chromatography and phosphocellulose column chromatography Candida albicans

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.19 ATP + RNAn = diphosphate + RNAn+3'-A initial interaction between RNA and the enzyme is characterized by a high enthalpy of association. The minimal RNA binding site of the enzyme is eight nucleotides. Upon RNA binding, the enzyme undergoes structural modifications, although the interaction does not significantly modify the stability of the protein Candida albicans
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.19 ATP + RNA
-
 Candida albicans diphosphate + RNA(A)n
-
 ?
2.7.7.19 additional information dATP is not used efficiently by the PAP protein, neither CTP nor UTP is an effective substrate, GTP is used at 6.3% of the rate of ATP Candida albicans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.19 PAP
-
 Candida albicans
2.7.7.19 poly(A) polymerase
-
 Candida albicans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.19 25 30
-
 Candida albicans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.19 50
-
 reactions incubated at 50°C result in a decrease of nearly 50% of incorporation Candida albicans