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Literature summary extracted from

  • Freistadt, M.S.; Vaccaro, J.A.; Eberle, K.E.
    Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase (2007), Virol. J., 4, 44.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.48 expressed in Escherichia coli BL21 (DE3) pCG1 cells Enterovirus C

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 heparin 100 nM heparin rapidly inactivates 3Dpol by inhibiting reinitiation Enterovirus C
2.7.7.48 NTP 3Dpol is inhibited by NTP concentrations higher than 2 mM Enterovirus C

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Enterovirus C
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48 Sepharose SPHP XK16/20 column chromatography, Q20 column chromatography, Q5 column chromatography, and UNO column chromatography Enterovirus C

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn
-
 Enterovirus C diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn RdRP incorporation of incorrect nucleosides is inefficient, making precise determination of kinetic parameters experimentally challenging. The fidelity for poliovirus polymerase 3Dpol ranges from 12000 to 1000000 for transition mutations and 32000 to 43000000 for transversion mutations Enterovirus C diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 3Dpol
-
 Enterovirus C
2.7.7.48 polymerase 3Dpol
-
 Enterovirus C
2.7.7.48 RDRP
-
 Enterovirus C
2.7.7.48 RNA-dependent RNA polymerase
-
 Enterovirus C