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Literature summary extracted from

  • de Gonzalo, G.; Torres Pazmino, D.E.; Ottolina, G.; Fraaije, M.W.; Carrea, G.
    Oxidations catalyzed by phenylacetone monooxygenase from Thermobifida fusca (2005), Tetrahedron, 16, 3077-3083.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.14.13.92 synthesis the enzyme has catalytic potential as a biocatalyst Thermobifida fusca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.92 additional information
-
 additional information steady-state kinetics Thermobifida fusca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.92 phenylacetone + NADPH + H+ + O2 Thermobifida fusca
-
 benzyl acetate + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.92 Thermobifida fusca
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.92 additional information
-
 substrate specificity, diverse substrates, overview Thermobifida fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.92 (R)-1-acetoxy-phenylacetone + NADPH + O2
-
 Thermobifida fusca (R)-1-hydroxy-1-phenylacetone + NADP+ + H2O
-
 ?
1.14.13.92 (S)-nicotine + NADPH + O2
-
 Thermobifida fusca ?
-
 ?
1.14.13.92 3-phenylpenta-2,4-dione + NADPH + O2
-
 Thermobifida fusca (R)-phenylacetylcarbinol + NADP+ + H2O the product is a well-known precursor in the synthesis of ephedrine and pseudoephedrine ?
1.14.13.92 benzylacetone + NADPH + O2 low activity Thermobifida fusca ?
-
 ?
1.14.13.92 diketone + NADPH + O2
-
 Thermobifida fusca (R)-1-acetoxy-phenylacetone + NADP+ + H2O
-
 ?
1.14.13.92 additional information the recombinant His-tagged enzyme is active with a large range of sulfides and ketones, as well as with several sulfoxides, an amine and an organoboron compound, high enantioselectivity dependeing on the substrate, substrate specificity, overview Thermobifida fusca ?
-
 ?
1.14.13.92 N,N-dimethylbenzylamine + NADPH + O2
-
 Thermobifida fusca N,N-dimethylbenzylamine N-oxide + NADP+ + H2O
-
 ?
1.14.13.92 phenylacetone + NADPH + H+ + O2
-
 Thermobifida fusca benzyl acetate + NADP+ + H2O
-
 ?
1.14.13.92 phenylboronic acid + NADPH + O2 formation of phenol Thermobifida fusca ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.92 More the enzyme belongs to the Baeyer-Villiger monooxygenases Thermobifida fusca
1.14.13.92 PAMO
-
 Thermobifida fusca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.92 25
-
 assay at Thermobifida fusca

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.92 additional information
-
 additional information all substrates show a turnover between 1.2 s-1 and 3.6 s-1 Thermobifida fusca

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.92 NADPH
-
 Thermobifida fusca