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Literature summary extracted from

  • Eprintsev, A.T.; Iventev, A.N.; Popov, V.N.
    Distribution and properties of glycolate oxidase from bundle sheath and mesophyll cells of green amaranth leaves (Amaranthus retroflecsus) (2005), Russ. J. Plant Physiol., 52, 553-558.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.15 isocitrate
-
 Amaranthus retroflexus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.15 succinate
-
 Amaranthus retroflexus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.15 0.02
-
 glycolate pH 7.5, mesophyll cell isozyme Amaranthus retroflexus
1.1.3.15 0.058
-
 glycolate pH 7.5, bundle sheath cell isozyme Amaranthus retroflexus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.15 peroxisome
-
 Amaranthus retroflexus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.15 37000
-
 x * 44000, bundle sheath cell isozyme, SDS-PAGE, x * 37000, mesophyll cell isozyme, SDS-PAGE Amaranthus retroflexus
1.1.3.15 44000
-
 x * 44000, bundle sheath cell isozyme, SDS-PAGE, x * 37000, mesophyll cell isozyme, SDS-PAGE Amaranthus retroflexus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.15 glycolate + O2 Amaranthus retroflexus
-
 glyoxylate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.15 Amaranthus retroflexus
-
 green amaranth
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.15 tissue-specific isozymes from leaves 63.5fold by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, bundle sheath cell isozyme and mesophyll cell isozyme to homogeneity Amaranthus retroflexus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.15 bundle sheath main location of the enzyme activity Amaranthus retroflexus
-
1.1.3.15 leaf from 18-days-old seedlings Amaranthus retroflexus
-
1.1.3.15 mesophyll
-
 Amaranthus retroflexus
-
1.1.3.15 additional information one isoform is located in bundle sheath cells, the second isozyme has dual location, and the third one is located in mesophyll cells Amaranthus retroflexus
-
1.1.3.15 seedling
-
 Amaranthus retroflexus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.15 0.55
-
 purified mesophyll cell isozyme Amaranthus retroflexus
1.1.3.15 0.62
-
 purified whole leaf enzyme Amaranthus retroflexus
1.1.3.15 1.33
-
 purified bundle sheath cell isozyme Amaranthus retroflexus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.15 glycolate + O2
-
 Amaranthus retroflexus glyoxylate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.15 ? x * 44000, bundle sheath cell isozyme, SDS-PAGE, x * 37000, mesophyll cell isozyme, SDS-PAGE Amaranthus retroflexus

Synonyms

EC Number Synonyms Comment Organism
1.1.3.15 glycolate oxidase
-
 Amaranthus retroflexus
1.1.3.15 GO
-
 Amaranthus retroflexus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.15 FMN
-
 Amaranthus retroflexus