BRENDA - Enzyme Database

Distribution and properties of glycolate oxidase from bundle sheath and mesophyll cells of green amaranth leaves (Amaranthus retroflecsus)

Eprintsev, A.T.; Iventev, A.N.; Popov, V.N.; Russ. J. Plant Physiol. 52, 553-558 (2005)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.3.15
isocitrate
-
Amaranthus retroflexus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.15
succinate
-
Amaranthus retroflexus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.15
0.02
-
glycolate
pH 7.5, mesophyll cell isozyme
Amaranthus retroflexus
1.1.3.15
0.058
-
glycolate
pH 7.5, bundle sheath cell isozyme
Amaranthus retroflexus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.3.15
peroxisome
-
Amaranthus retroflexus
5777
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.15
37000
-
x * 44000, bundle sheath cell isozyme, SDS-PAGE, x * 37000, mesophyll cell isozyme, SDS-PAGE
Amaranthus retroflexus
1.1.3.15
44000
-
x * 44000, bundle sheath cell isozyme, SDS-PAGE, x * 37000, mesophyll cell isozyme, SDS-PAGE
Amaranthus retroflexus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.3.15
glycolate + O2
Amaranthus retroflexus
-
glyoxylate + H2O2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.3.15
Amaranthus retroflexus
-
green amaranth
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.3.15
tissue-specific isozymes from leaves 63.5fold by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, bundle sheath cell isozyme and mesophyll cell isozyme to homogeneity
Amaranthus retroflexus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.3.15
bundle sheath
main location of the enzyme activity
Amaranthus retroflexus
-
1.1.3.15
leaf
from 18-days-old seedlings
Amaranthus retroflexus
-
1.1.3.15
mesophyll
-
Amaranthus retroflexus
-
1.1.3.15
additional information
one isoform is located in bundle sheath cells, the second isozyme has dual location, and the third one is located in mesophyll cells
Amaranthus retroflexus
-
1.1.3.15
seedling
-
Amaranthus retroflexus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.3.15
0.55
-
purified mesophyll cell isozyme
Amaranthus retroflexus
1.1.3.15
0.62
-
purified whole leaf enzyme
Amaranthus retroflexus
1.1.3.15
1.33
-
purified bundle sheath cell isozyme
Amaranthus retroflexus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.15
glycolate + O2
-
677109
Amaranthus retroflexus
glyoxylate + H2O2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.3.15
?
x * 44000, bundle sheath cell isozyme, SDS-PAGE, x * 37000, mesophyll cell isozyme, SDS-PAGE
Amaranthus retroflexus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.15
FMN
-
Amaranthus retroflexus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.3.15
isocitrate
-
Amaranthus retroflexus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.15
FMN
-
Amaranthus retroflexus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.3.15
succinate
-
Amaranthus retroflexus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.15
0.02
-
glycolate
pH 7.5, mesophyll cell isozyme
Amaranthus retroflexus
1.1.3.15
0.058
-
glycolate
pH 7.5, bundle sheath cell isozyme
Amaranthus retroflexus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.3.15
peroxisome
-
Amaranthus retroflexus
5777
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.15
37000
-
x * 44000, bundle sheath cell isozyme, SDS-PAGE, x * 37000, mesophyll cell isozyme, SDS-PAGE
Amaranthus retroflexus
1.1.3.15
44000
-
x * 44000, bundle sheath cell isozyme, SDS-PAGE, x * 37000, mesophyll cell isozyme, SDS-PAGE
Amaranthus retroflexus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.3.15
glycolate + O2
Amaranthus retroflexus
-
glyoxylate + H2O2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.3.15
tissue-specific isozymes from leaves 63.5fold by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, bundle sheath cell isozyme and mesophyll cell isozyme to homogeneity
Amaranthus retroflexus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.3.15
bundle sheath
main location of the enzyme activity
Amaranthus retroflexus
-
1.1.3.15
leaf
from 18-days-old seedlings
Amaranthus retroflexus
-
1.1.3.15
mesophyll
-
Amaranthus retroflexus
-
1.1.3.15
additional information
one isoform is located in bundle sheath cells, the second isozyme has dual location, and the third one is located in mesophyll cells
Amaranthus retroflexus
-
1.1.3.15
seedling
-
Amaranthus retroflexus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.3.15
0.55
-
purified mesophyll cell isozyme
Amaranthus retroflexus
1.1.3.15
0.62
-
purified whole leaf enzyme
Amaranthus retroflexus
1.1.3.15
1.33
-
purified bundle sheath cell isozyme
Amaranthus retroflexus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.15
glycolate + O2
-
677109
Amaranthus retroflexus
glyoxylate + H2O2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.3.15
?
x * 44000, bundle sheath cell isozyme, SDS-PAGE, x * 37000, mesophyll cell isozyme, SDS-PAGE
Amaranthus retroflexus