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Literature summary extracted from

  • Wang, L.K.; Nandakumar, J.; Schwer, B.; Shuman, S.
    The C-terminal domain of T4 RNA ligase 1 confers specificity for tRNA repair (2007), RNA, 13, 1235-1244.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.3 H250A pRNA circularization by the mutant enzyme is 6% of the wild-type rate Tequatrovirus T4
6.5.1.3 K117A mutation is lethal in vivo Tequatrovirus T4
6.5.1.3 L104A L104A strain is temperature sensitive, no growth at 37°C Tequatrovirus T4
6.5.1.3 L104A pRNA circularization by the mutant enzyme is 6% of the wild-type rate Tequatrovirus T4
6.5.1.3 additional information S121A, S124A, and H250 strains grow at all temperatures Tequatrovirus T4
6.5.1.3 S103A pRNA circularization by the mutant enzyme is 5% of the wild-type rate Tequatrovirus T4
6.5.1.3 S103A S103A strain is temperature sensitive, no growth at 37°C Tequatrovirus T4
6.5.1.3 S118A S118 strain is temperature sensitive, no growth at 37°C Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.3 Tequatrovirus T4
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.3 ATP + (ribonucleotide)n + (ribonucleotide)m Rnl1 has an inherent specificity for sealing tRNA with a break in the anticodon loop. The tRNA specificity is imparted by the C domain Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.3 RNA ligase 1
-
 Tequatrovirus T4
6.5.1.3 Rnl1
-
 Tequatrovirus T4