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Literature summary extracted from

  • Rosen, A.E.; Brooks, B.S.; Guth, E.; Francklyn, C.S.; Musier-Forsyth, K.
    Evolutionary conservation of a functionally important backbone phosphate group critical for aminoacylation of histidine tRNAs (2006), RNA, 12, 1315-1322.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.21 additional information mutational study of the 1:73 base pair recognition by yeats HisRS using two chemically synthesized 24-nucleotide RNA microhelices, overview Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.21 0.00014
-
 tRNAHis pH 8.0, 37°C, aminoacylation Saccharomyces cerevisiae
6.1.1.21 0.0038
-
 RNA microhelix pH 8.0, 37°C, aminoacylation Escherichia coli
6.1.1.21 0.009
-
 L-histidine pH 8.0, 37°C, diphosphate exchange Saccharomyces cerevisiae
6.1.1.21 0.03
-
 L-histidine pH 8.0, 37°C, diphosphate exchange Escherichia coli
6.1.1.21 0.034
-
 tRNAHis pH 8.0, 37°C, aminoacylation Escherichia coli
6.1.1.21 0.124
-
 RNA microhelix pH 8.0, 37°C, aminoacylation Saccharomyces cerevisiae
6.1.1.21 0.5
-
 ATP pH 8.0, 37°C, diphosphate exchange Saccharomyces cerevisiae
6.1.1.21 0.89
-
 ATP pH 8.0, 37°C, diphosphate exchange Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.21 Mg2+
-
 Escherichia coli
6.1.1.21 Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis Escherichia coli
-
 AMP + diphosphate + L-histidinyl-tRNAHis
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis Saccharomyces cerevisiae
-
 AMP + diphosphate + L-histidinyl-tRNAHis
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.21 Escherichia coli
-
-
-
6.1.1.21 Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.21 additional information
-
-
 Escherichia coli
6.1.1.21 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.21 ATP + L-histidine + RNA microhelix
-
 Escherichia coli ?
-
 ?
6.1.1.21 ATP + L-histidine + RNA microhelix
-
 Saccharomyces cerevisiae ?
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Escherichia coli AMP + diphosphate + L-histidinyl-tRNAHis
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Saccharomyces cerevisiae AMP + diphosphate + L-histidinyl-tRNAHis
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis all histidine tRNA molecules have an extra nucleotide, G-1, at the 59 end of the acceptor stem, G-1:C73 base pair binding analysis of the yeast enzyme Saccharomyces cerevisiae AMP + diphosphate + L-histidinyl-tRNAHis
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis all histidine tRNA molecules have an extra nucleotide, G-1, at the 59 end of the acceptor stem, importance of the G-1:C73 base pair to tRNAHisc, identity, specifically the 59-monophosphate of G-1 and the major groove amine of C73 are recognized by the enzyme of Escherichia coli stabilizing the reaction transition state Escherichia coli AMP + diphosphate + L-histidinyl-tRNAHis
-
 ?
6.1.1.21 additional information binding of a chemically synthesized 24-nucleotide RNA microhelix, which recapitulates the acceptor stem of Escherichia coli tRNAHis, functional group analysis, overview Escherichia coli ?
-
 ?
6.1.1.21 additional information binding of a chemically synthesized 24-nucleotide RNA microhelix, which recapitulates the acceptor stem of Saccharomyces cervisiae tRNAHis, functional group analysis, overview Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.21 HisRS
-
 Escherichia coli
6.1.1.21 HisRS
-
 Saccharomyces cerevisiae
6.1.1.21 histidyl-transfer RNA synthetase
-
 Escherichia coli
6.1.1.21 histidyl-transfer RNA synthetase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.21 30
-
 aminoacylation assay at Escherichia coli
6.1.1.21 30
-
 aminoacylation assay at Saccharomyces cerevisiae
6.1.1.21 37
-
 diphosphate exchange assay at Escherichia coli
6.1.1.21 37
-
 diphosphate exchange assay at Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.21 0.0013
-
 RNA microhelix pH 8.0, 37°C, aminoacylation Saccharomyces cerevisiae
6.1.1.21 0.024
-
 RNA microhelix pH 8.0, 37°C, aminoacylation Escherichia coli
6.1.1.21 0.42
-
 tRNAHis pH 8.0, 37°C, aminoacylation Saccharomyces cerevisiae
6.1.1.21 1.71
-
 tRNAHis pH 8.0, 37°C, aminoacylation Escherichia coli
6.1.1.21 40
-
 ATP pH 8.0, 37°C, diphosphate exchange Saccharomyces cerevisiae
6.1.1.21 40
-
 L-histidine pH 8.0, 37°C, diphosphate exchange Saccharomyces cerevisiae
6.1.1.21 130
-
 ATP pH 8.0, 37°C, diphosphate exchange Escherichia coli
6.1.1.21 130
-
 L-histidine pH 8.0, 37°C, diphosphate exchange Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.21 7.5
-
 aminoacylation assay at Escherichia coli
6.1.1.21 7.5
-
 aminoacylation assay at Saccharomyces cerevisiae
6.1.1.21 8
-
 diphosphate exchange assay at Escherichia coli
6.1.1.21 8
-
 diphosphate exchange assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.21 ATP
-
 Escherichia coli
6.1.1.21 ATP
-
 Saccharomyces cerevisiae