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Literature summary extracted from

  • Bonatto, A.C.; Couto, G.H.; Souza, E.M.; Araujo, L.M.; Pedrosa, F.O.; Noindorf, L.; Benelli, E.M.
    Purification and characterization of the bifunctional uridylyltransferase and the signal transducing proteins GlnB and GlnK from Herbaspirillum seropedicae (2007), Protein Expr. Purif., 55, 293-299.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.59 2-oxoglutarate required compound, uridylylation assay with 5 mM 2-oxoglutarate Herbaspirillum seropedicae
2.7.7.59 ATP required compound, uridylylation assay with 0.2 mM ATP Herbaspirillum seropedicae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.59 expression in Echerichia coli RB9065lambdaDE3 Herbaspirillum seropedicae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.59 glutamine shows an inhibition of 65% of GlnB uridylylation and 70% of GlnK uridylylation Herbaspirillum seropedicae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.59 K+ uridylylation assay with 100 mM KCl Herbaspirillum seropedicae
2.7.7.59 Mg2+ uridylylation assay with 25 mM Mg2+ Herbaspirillum seropedicae

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.59 Herbaspirillum seropedicae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.59 GlnD is less soluble than the purified PII proteins GlnB or GlnK, to improve solubility of GlnD a special buffer is utilised (50 mM Tris-HCl, pH 7.0, 200 mM NaCl, 1 mM DTT) which produces 50% solubilization Herbaspirillum seropedicae
2.7.7.59 protein is purified by anionic exchange chromatography (DEAE-Sepharose column) and gel chromatography (Agarose-Heparin column) Herbaspirillum seropedicae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.59 UTP + [protein-PII] GlnD of Herbaspirillum seropedicae is overexpressed with the two PII proteins GlnK and GlnB. Results show that GlnD uridylylates GlnB and GlnK trimers producing the forms PII(UMP)1, PII(UMP)2 and PII(UMP)3. GlnB is more efficiently uridylylated than GlnK Herbaspirillum seropedicae diphosphate + uridylyl-[protein-PII]
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.7.59 GlnD
-
 Herbaspirillum seropedicae
2.7.7.59 uridylyltransferase
-
 Herbaspirillum seropedicae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.59 7.5
-
 assay at Herbaspirillum seropedicae