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Literature summary extracted from

  • Claeyssen, E.; Wally, O.; Matton, D.P.; Morse, D.; Rivoal, J.
    Cloning, expression, purification, and properties of a putative plasma membrane hexokinase from Solanum chacoense (2006), Protein Expr. Purif., 47, 329-339.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.1 expressed as a hexahistidine-tagged protein in Escherichia coli Solanum chacoense

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.1 ADP 0.5 mM, 43% inhibition Solanum chacoense
2.7.1.1 D-glucosamine 20 mM, 67% inhibition Solanum chacoense
2.7.1.1 D-mannose 10 mM, 88% loss of activity Solanum chacoense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.1 0.023
-
 D-glucose 30°C, pH 8.0 Solanum chacoense
2.7.1.1 0.03
-
 D-mannose 30°C, pH 8.0 Solanum chacoense
2.7.1.1 0.061
-
 ATP 30°C, pH 8.0 Solanum chacoense
2.7.1.1 0.231
-
 GTP 30°C, pH 8.0 Solanum chacoense
2.7.1.1 0.231
-
 TTP 30°C, pH 8.0 Solanum chacoense
2.7.1.1 2
-
 CTP 30°C, pH 8.0 Solanum chacoense
2.7.1.1 2.2
-
 UTP 30°C, pH 8.0 Solanum chacoense
2.7.1.1 5.2
-
 D-fructose 30°C, pH 8.0 Solanum chacoense

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.1 plasma membrane
-
 Solanum chacoense 5886
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.1 Solanum chacoense Q3S2I3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.1 full length recombinant protein Solanum chacoense

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.1 5.3
-
-
 Solanum chacoense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.1 ATP + D-fructose
-
 Solanum chacoense ADP + D-fructose 6-phosphate
-
 ?
2.7.1.1 ATP + D-glucose
-
 Solanum chacoense ADP + D-glucose 6-phosphate
-
 ?
2.7.1.1 ATP + D-mannose
-
 Solanum chacoense ADP + D-mannose 6-phosphate
-
 ?
2.7.1.1 CTP + D-glucose
-
 Solanum chacoense CDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 GTP + D-glucose
-
 Solanum chacoense GDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 TTP + D-glucose
-
 Solanum chacoense TDP + D-glucose 6-phosphate
-
 ?
2.7.1.1 UTP + D-glucose
-
 Solanum chacoense UDP + D-glucose 6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.1 ScHK2
-
 Solanum chacoense

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.1 0.85
-
 GTP 30°C, pH 8.0 Solanum chacoense
2.7.1.1 1.42
-
 TTP 30°C, pH 8.0 Solanum chacoense
2.7.1.1 1.61
-
 CTP 30°C, pH 8.0 Solanum chacoense
2.7.1.1 3.02
-
 UTP 30°C, pH 8.0 Solanum chacoense
2.7.1.1 3.3
-
 D-glucose 30°C, pH 8.0 Solanum chacoense
2.7.1.1 3.5
-
 ATP 30°C, pH 8.0 Solanum chacoense
2.7.1.1 3.69
-
 D-fructose 30°C, pH 8.0 Solanum chacoense
2.7.1.1 3.78
-
 D-mannose 30°C, pH 8.0 Solanum chacoense
2.7.1.1 5.01
-
 D-glucose 30°C, pH 8.0 Solanum chacoense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.1 8.7
-
-
 Solanum chacoense

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.1 7.7 8.7 ScHK2 activity decreases sharply (3fold) within a narrow range from pH 8.7 down to pH 7.7 Solanum chacoense