EC Number | Application | Comment | Organism |
---|---|---|---|
1.13.12.5 | molecular biology | the development of variants of Renilla luciferase, which exhibit significantly improved properties compared with the native enzyme, will allow enhanced sensitivity in existing luciferase-based assays as well as enable the development of novel probes labeled with the luciferase protein | Renilla sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.12.5 | A55T/C124A/S130A/K136R/A143M/M185V/M253L/S287L | mutant enzyme exhibits a greater than 4-fold enhancement in activity, a 200fold increased resistance to serum inactivation, and a small but measurable 5 nm red shift in the emission spectrum. The enhancement in light output arises from a combination of increases in quantum yield and improvedkinetics | Renilla sp. |
1.13.12.5 | M185V/Q235A | compared with the native enzyme the mutant has twice the rate of inactivation, as measured in murine serum, while incorporating a close to 5fold improvement in light output | Renilla sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.12.5 | 0.0016 | - |
coelenterazine | mutant enzyme A55T/C124A/S130A/K136R/A143M/M185V/M253L/S287L | Renilla sp. | |
1.13.12.5 | 0.0029 | - |
coelenterazine | wild-type enzyme | Renilla sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.13.12.5 | additional information | recombinant enzyme is produced from a periplasmically or cytoplasmically expressed expression plasmid in Escherichia coli | Renilla sp. | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.12.5 | 33800 | - |
1 * 33800, mutant enzymeA55T/C124A/S130A/K136R/A143M/M185V/M253L/S287L, molar mass moment calculations based on the multi-angle scattering | Renilla sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.12.5 | Renilla sp. | - |
recombinant | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.5 | coelenterazine + O2 | - |
Renilla sp. | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.12.5 | monomer | 1 * 33800, mutant enzymeA55T/C124A/S130A/K136R/A143M/M185V/M253L/S287L, molar mass moment calculations based on the multi-angle scattering | Renilla sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.12.5 | RLuc | - |
Renilla sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.12.5 | 3.9 | - |
coelenterazine | wild-type enzyme | Renilla sp. | |
1.13.12.5 | 4.9 | - |
coelenterazine | mutant enzyme A55T/C124A/S130A/K136R/A143M/M185V/M253L/S287L | Renilla sp. |