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Literature summary extracted from

  • Forouhar, F.; Anderson, J.L.; Mowat, C.G.; Vorobiev, S.M.; Hussain, A.; Abashidze, M.; Bruckmann, C.; Thackray, S.J.; Seetharaman, J.; Tucker, T.; Xiao, R.; Ma, L.C.; Zhao, L.; Acton, T.B.; Montelione, G.T.; Chapman, S.K.; Tong, L.
    Molecular insights into substrate recognition and catalysis by tryptophan 2,3-dioxygenase (2007), Proc. Natl. Acad. Sci. USA, 104, 473-478.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.52 full-length enzyme is cloned into a pET-21d derivative, with a C-terminal hexahistidine tag, and overexpressed at 17°C in Escherichia coli BL21(DE3) pMGK cells Xanthomonas campestris

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.52 sitting-drop vapor diffusion method, crystal structures at up to 1.6-A resolution of the active, reduced (Fe(II)) enzyme in a binary complex with the substrate L-Trp or 6-fluoro-Trp Xanthomonas campestris

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.52 H55A kcat for L-Trp is 6.8fold lower than wild-type enzyme, KM-value is 1.7fold higher than wild-type value Xanthomonas campestris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.52 0.114
-
 L-Trp 25°C, pH 7.5, wild-type enzyme Xanthomonas campestris
1.13.11.52 100
-
 5-fluoro-DL-tryptophan 25°C, pH 7.5, wild-type enzyme Xanthomonas campestris
1.13.11.52 119
-
 O2 25°C, pH 7.5, wild-type enzyme Xanthomonas campestris
1.13.11.52 133
-
 L-Trp 25°C, pH 7.5, mutant enzyme H55A Xanthomonas campestris
1.13.11.52 186
-
 6-fluoro-DL-tryptophan 25°C, pH 7.5, wild-type enzyme Xanthomonas campestris

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.52 Xanthomonas campestris Q8PDA8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.52
-
 Xanthomonas campestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.52 5-fluoro-DL-tryptophan + O2
-
 Xanthomonas campestris ?
-
 ?
1.13.11.52 6-fluoro-DL-tryptophan + O2
-
 Xanthomonas campestris ?
-
 ?
1.13.11.52 L-Trp + O2
-
 Xanthomonas campestris N-formyl-L-kynurenine
-
 ?
1.13.11.52 additional information no activity with D-Trp and indolepropionic acid Xanthomonas campestris ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.52 TDO
-
 Xanthomonas campestris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.52 2.4
-
 5-fluoro-DL-tryptophan 25°C, pH 7.5, wild-type enzyme Xanthomonas campestris
1.13.11.52 2.86
-
 L-Trp 25°C, pH 7.5, mutant enzyme H55A Xanthomonas campestris
1.13.11.52 3 6 L-Trp 25°C, pH 7.5, mutant enzyme H55A Xanthomonas campestris
1.13.11.52 19.5
-
 L-Trp 25°C, pH 7.5, wild-type enzyme Xanthomonas campestris
1.13.11.52 35.4
-
 O2 25°C, pH 7.5, wild-type enzyme Xanthomonas campestris
1.13.11.52 37.3
-
 6-fluoro-DL-tryptophan 25°C, pH 7.5, wild-type enzyme Xanthomonas campestris

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.52 heme
-
 Xanthomonas campestris