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Literature summary extracted from

  • Dai, N.; Petreikov, M.; Portnoy, V.; Katzir, N.; Pharr, D.M.; Schaffer, A.A.
    Cloning and expression analysis of a UDP-galactose/glucose pyrophosphorylase from melon fruit provides evidence for the major metabolic pathway of galactose metabolism in raffinose oligosaccharide metabolizing plants (2006), Plant Physiol., 142, 294-304.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.9
-
 Cucumis melo
2.7.7.64 expressed in Escherichia coli Cucumis melo

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.9 0.11
-
 UDP-glucose pH 7.5, 30°C Cucumis melo
2.7.7.9 0.24
-
 alpha-D-glucose 1-phosphate pH 7.5, 30°C Cucumis melo
2.7.7.9 0.26
-
 UDP-galactose pH 7.5, 30°C Cucumis melo
2.7.7.64 0.14
-
 UDP-glucose
-
 Cucumis melo
2.7.7.64 0.27
-
 alpha-D-glucose 1-phosphate
-
 Cucumis melo
2.7.7.64 0.43
-
 alpha-D-galactose 1-phosphate
-
 Cucumis melo
2.7.7.64 0.44
-
 UDP-galactose
-
 Cucumis melo

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.64 68000
-
 SDS-PAGE Cucumis melo

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Cucumis melo Q19TV8 in addition to UGPase, presence of dual substrate UDP-hexose pyrophosphorylase
-
2.7.7.64 Cucumis melo Q0GZS3 group Reticulatus, cultivar Noy Yizre’el
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.9
-
 Cucumis melo
2.7.7.64 MonoQ HR 5/5 column chromatography Cucumis melo

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.9 fruit expression throughout fruit development Cucumis melo
-
2.7.7.64 fruit
-
 Cucumis melo
-
2.7.7.64 ovary
-
 Cucumis melo
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.64 223
-
 with UDP-glucose as a substrate Cucumis melo
2.7.7.64 233
-
 with alpha-D-galactose 1-phosphate as a substrate Cucumis melo
2.7.7.64 410
-
 with alpha-D-glucose 1-phosphate as a substrate Cucumis melo
2.7.7.64 678
-
 with UDP-galactose as a substrate Cucumis melo

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 diphosphate + UDP-galactose
-
 Cucumis melo UTP + alpha-D-galactose 1-phosphate
-
 r
2.7.7.9 diphosphate + UDP-glucose
-
 Cucumis melo UTP + alpha-D-glucose 1-phosphate
-
 r
2.7.7.9 additional information no substrate: alpha-D-glalctose 1-phosphate Cucumis melo ?
-
 ?
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Cucumis melo diphosphate + UDP-glucose
-
 r
2.7.7.64 UTP + alpha-D-galactose 1-phosphate
-
 Cucumis melo diphosphate + UDP-galactose
-
 r
2.7.7.64 UTP + alpha-D-glucose 1-phosphate
-
 Cucumis melo diphosphate + UDP-glucose
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.7.64 UDP-Gal/Glc PPase
-
 Cucumis melo
2.7.7.64 UDP-galactose/glucose pyrophosphorylase
-
 Cucumis melo
2.7.7.64 UGGPase
-
 Cucumis melo