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Literature summary extracted from

  • Saha, S.; Enugutti, B.; Rajakumari, S.; Rajasekharan, R.
    Cytosolic triacylglycerol biosynthetic pathway in oilseeds. Molecular cloning and expression of peanut cytosolic diacylglycerol acyltransferase (2006), Plant Physiol., 141, 1533-1543.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 expression as His-tagged enzyme in Escherichia coli strain BL21 (DE3), phylogenetic analysis Arachis hypogaea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 cytosol
-
 Arachis hypogaea 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.20 Mg2+
-
 Arachis hypogaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.20 41000
-
 x * 41000, recombinant His-tagged enzyme, SDS-PAGE Arachis hypogaea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 1,2-diacylglycerol + acyl-CoA Arachis hypogaea the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA Arachis hypogaea JL 24 the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols triacylglycerol + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Arachis hypogaea
-
-
-
2.3.1.20 Arachis hypogaea Q2KP14
-
-
2.3.1.20 Arachis hypogaea JL 24 Q2KP14
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.20 native enzyme 1724fold from developing cotyldons by hydrophobic interaction and adsorption chromatography, followed by gel filtration and heparin affinity chromatography to homogeneity Arachis hypogaea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 cotyledon developing Arachis hypogaea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.20 additional information
-
 substrate specificity of native and recombinant enzyme Arachis hypogaea
2.3.1.20 0.0071
-
 purified native enzyme Arachis hypogaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 1,2-diacylglycerol + acyl-CoA the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols Arachis hypogaea triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-diacylglycerol + acyl-CoA the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols Arachis hypogaea JL 24 triacylglycerol + CoA
-
 ?
2.3.1.20 1,2-dipalmitoyl-sn-glycerol + oleoyl-CoA
-
 Arachis hypogaea 1,2-dipalmitoyl-3-oleoyl-sn-glycerol + CoA
-
 ?
2.3.1.20 1,2-dipalmitoyl-sn-glycerol + palmitoyl-CoA
-
 Arachis hypogaea tripalmitin + CoA
-
 ?
2.3.1.20 1,2-dipalmitoyl-sn-glycerol + stearoyl-CoA
-
 Arachis hypogaea 1,2-dipalmitoyl-3-stearoyl-sn-glycerol + CoA
-
 ?
2.3.1.20 additional information substrate specificity of native and recombinant enzyme, the native and the recombinant enzyme do not show wax ester synthase activity in contrast to other DGATs, no activity with hexadecanol, glycerol-3-phosphate, monoacylglycerol, lysophosphatidic acid, and lysophosphatidylcholine, oleoyl-CoA is the preferred acyl donor as compared to palmitoyl- and stearoyl-CoAs Arachis hypogaea ?
-
 ?
2.3.1.20 additional information substrate specificity of native and recombinant enzyme, the native and the recombinant enzyme do not show wax ester synthase activity in contrast to other DGATs, no activity with hexadecanol, glycerol-3-phosphate, monoacylglycerol, lysophosphatidic acid, and lysophosphatidylcholine, oleoyl-CoA is the preferred acyl donor as compared to palmitoyl- and stearoyl-CoAs Arachis hypogaea JL 24 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.20 ? x * 41000, recombinant His-tagged enzyme, SDS-PAGE Arachis hypogaea

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 acyl-CoA:diacylglycerol acyltransferase
-
 Arachis hypogaea
2.3.1.20 DGAT
-
 Arachis hypogaea
2.3.1.20 diacylglycerol acyltransferase
-
 Arachis hypogaea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.20 30
-
 assay at Arachis hypogaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.20 7.5
-
 assay at Arachis hypogaea