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Literature summary extracted from

  • Tavladoraki, P.; Rossi, M.N.; Saccuti, G.; Perez-Amador, M.A.; Polticelli, F.; Angelini, R.; Federico, R.
    Heterologous expression and biochemical characterization of a polyamine oxidase from Arabidopsis involved in polyamine back conversion (2006), Plant Physiol., 141, 1519-1532.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.16 AtPAO1 cDNA is isolated and cloned in a vector for heterologous expression in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.16 1,12-diaminododecane
-
 Arabidopsis thaliana
1.5.3.16 1,8-diaminooctane
-
 Arabidopsis thaliana
1.5.3.16 agmatine
-
 Arabidopsis thaliana
1.5.3.16 guazatine
-
 Arabidopsis thaliana
1.5.3.16 MDL72527
-
 Arabidopsis thaliana
1.5.3.16 N-prenylagmatine
-
 Arabidopsis thaliana
1.5.3.16 spermidine
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.16 0.09
-
 norspermine pH 8.0 Arabidopsis thaliana
1.5.3.16 0.11
-
 spermine pH 8.0 Arabidopsis thaliana
1.5.3.16 0.47
-
 N1-acetylspermine pH 8.0 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.16 additional information Arabidopsis thaliana no activity with spermidine ?
-
 ?
1.5.3.16 spermine + O2 + H2O Arabidopsis thaliana enzyme is involved in a polyamine back-conversion pathway spermidine + 3-aminopropanal + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.16 Arabidopsis thaliana Q9FNA2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.16 recombinant protein Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.16 additional information no activity with spermidine Arabidopsis thaliana ?
-
 ?
1.5.3.16 N1-acetylspermine + O2 + H2O
-
 Arabidopsis thaliana spermidine + 3-acetamidopropanal + H2O2
-
 ?
1.5.3.16 norspermine + O2 + H2O
-
 Arabidopsis thaliana ?
-
 ?
1.5.3.16 spermine + O2 + H2O
-
 Arabidopsis thaliana spermidine + 3-aminopropanal + H2O2
-
 ?
1.5.3.16 spermine + O2 + H2O enzyme is involved in a polyamine back-conversion pathway Arabidopsis thaliana spermidine + 3-aminopropanal + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.3.16 AtPAO1
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.16 0.2
-
 N1-acetylspermine pH 8.0 Arabidopsis thaliana
1.5.3.16 2.7
-
 spermine pH 8.0 Arabidopsis thaliana
1.5.3.16 6.9
-
 norspermine pH 8.0 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.16 8
-
 substrate: spermine or norspermine Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.3.16 7 9 pH 7: about 60% of maximal activity, pH 9.0: about 60% of maximal activity, substrate: spermine Arabidopsis thaliana
1.5.3.16 7 9 pH 7: about 80% of maximal activity, pH 9.0: about 60% of maximal activity, substrate: norspermine Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.16 FAD all FAD molecules in the purified enzyme are catalytically active Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.3.16 0.0007
-
 guazatine pH 8.0, substrate: spermine Arabidopsis thaliana
1.5.3.16 0.004
-
 N-prenylagmatine pH 8.0, substrate: spermine Arabidopsis thaliana
1.5.3.16 0.0117
-
 1,12-diaminododecane pH 8.0, substrate: spermine Arabidopsis thaliana
1.5.3.16 0.1373
-
 MDL72527 pH 8.0, substrate: spermine Arabidopsis thaliana
1.5.3.16 0.147
-
 agmatine pH 8.0, substrate: spermine Arabidopsis thaliana
1.5.3.16 0.293
-
 1,8-diaminooctane pH 8.0, substrate: spermine Arabidopsis thaliana
1.5.3.16 0.546
-
 spermidine pH 8.0, substrate: spermine Arabidopsis thaliana