BRENDA - Enzyme Database

Heterologous expression and biochemical characterization of a polyamine oxidase from Arabidopsis involved in polyamine back conversion

Tavladoraki, P.; Rossi, M.N.; Saccuti, G.; Perez-Amador, M.A.; Polticelli, F.; Angelini, R.; Federico, R.; Plant Physiol. 141, 1519-1532 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.5.3.16
AtPAO1 cDNA is isolated and cloned in a vector for heterologous expression in Escherichia coli
Arabidopsis thaliana
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.5.3.16
1,12-diaminododecane
-
Arabidopsis thaliana
1.5.3.16
1,8-diaminooctane
-
Arabidopsis thaliana
1.5.3.16
agmatine
-
Arabidopsis thaliana
1.5.3.16
guazatine
-
Arabidopsis thaliana
1.5.3.16
MDL72527
-
Arabidopsis thaliana
1.5.3.16
N-prenylagmatine
-
Arabidopsis thaliana
1.5.3.16
spermidine
-
Arabidopsis thaliana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.5.3.16
0.09
-
norspermine
pH 8.0
Arabidopsis thaliana
1.5.3.16
0.11
-
spermine
pH 8.0
Arabidopsis thaliana
1.5.3.16
0.47
-
N1-acetylspermine
pH 8.0
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.5.3.16
additional information
Arabidopsis thaliana
no activity with spermidine
?
-
-
-
1.5.3.16
spermine + O2 + H2O
Arabidopsis thaliana
enzyme is involved in a polyamine back-conversion pathway
spermidine + 3-aminopropanal + H2O2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.5.3.16
Arabidopsis thaliana
Q9FNA2
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.5.3.16
recombinant protein
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.3.16
additional information
no activity with spermidine
676604
Arabidopsis thaliana
?
-
-
-
-
1.5.3.16
N1-acetylspermine + O2 + H2O
-
676604
Arabidopsis thaliana
spermidine + 3-acetamidopropanal + H2O2
-
-
-
?
1.5.3.16
norspermine + O2 + H2O
-
676604
Arabidopsis thaliana
?
-
-
-
?
1.5.3.16
spermine + O2 + H2O
-
676604
Arabidopsis thaliana
spermidine + 3-aminopropanal + H2O2
-
-
-
?
1.5.3.16
spermine + O2 + H2O
enzyme is involved in a polyamine back-conversion pathway
676604
Arabidopsis thaliana
spermidine + 3-aminopropanal + H2O2
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.5.3.16
0.2
-
N1-acetylspermine
pH 8.0
Arabidopsis thaliana
1.5.3.16
2.7
-
spermine
pH 8.0
Arabidopsis thaliana
1.5.3.16
6.9
-
norspermine
pH 8.0
Arabidopsis thaliana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.5.3.16
8
-
substrate: spermine or norspermine
Arabidopsis thaliana
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.5.3.16
7
9
pH 7: about 60% of maximal activity, pH 9.0: about 60% of maximal activity, substrate: spermine; pH 7: about 80% of maximal activity, pH 9.0: about 60% of maximal activity, substrate: norspermine
Arabidopsis thaliana
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.5.3.16
FAD
all FAD molecules in the purified enzyme are catalytically active
Arabidopsis thaliana
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.5.3.16
0.0007
-
guazatine
pH 8.0, substrate: spermine
Arabidopsis thaliana
1.5.3.16
0.004
-
N-prenylagmatine
pH 8.0, substrate: spermine
Arabidopsis thaliana
1.5.3.16
0.0117
-
1,12-diaminododecane
pH 8.0, substrate: spermine
Arabidopsis thaliana
1.5.3.16
0.1373
-
MDL72527
pH 8.0, substrate: spermine
Arabidopsis thaliana
1.5.3.16
0.147
-
agmatine
pH 8.0, substrate: spermine
Arabidopsis thaliana
1.5.3.16
0.293
-
1,8-diaminooctane
pH 8.0, substrate: spermine
Arabidopsis thaliana
1.5.3.16
0.546
-
spermidine
pH 8.0, substrate: spermine
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.5.3.16
AtPAO1 cDNA is isolated and cloned in a vector for heterologous expression in Escherichia coli
Arabidopsis thaliana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.5.3.16
FAD
all FAD molecules in the purified enzyme are catalytically active
Arabidopsis thaliana
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.5.3.16
1,12-diaminododecane
-
Arabidopsis thaliana
1.5.3.16
1,8-diaminooctane
-
Arabidopsis thaliana
1.5.3.16
agmatine
-
Arabidopsis thaliana
1.5.3.16
guazatine
-
Arabidopsis thaliana
1.5.3.16
MDL72527
-
Arabidopsis thaliana
1.5.3.16
N-prenylagmatine
-
Arabidopsis thaliana
1.5.3.16
spermidine
-
Arabidopsis thaliana
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.5.3.16
0.0007
-
guazatine
pH 8.0, substrate: spermine
Arabidopsis thaliana
1.5.3.16
0.004
-
N-prenylagmatine
pH 8.0, substrate: spermine
Arabidopsis thaliana
1.5.3.16
0.0117
-
1,12-diaminododecane
pH 8.0, substrate: spermine
Arabidopsis thaliana
1.5.3.16
0.1373
-
MDL72527
pH 8.0, substrate: spermine
Arabidopsis thaliana
1.5.3.16
0.147
-
agmatine
pH 8.0, substrate: spermine
Arabidopsis thaliana
1.5.3.16
0.293
-
1,8-diaminooctane
pH 8.0, substrate: spermine
Arabidopsis thaliana
1.5.3.16
0.546
-
spermidine
pH 8.0, substrate: spermine
Arabidopsis thaliana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.5.3.16
0.09
-
norspermine
pH 8.0
Arabidopsis thaliana
1.5.3.16
0.11
-
spermine
pH 8.0
Arabidopsis thaliana
1.5.3.16
0.47
-
N1-acetylspermine
pH 8.0
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.5.3.16
additional information
Arabidopsis thaliana
no activity with spermidine
?
-
-
-
1.5.3.16
spermine + O2 + H2O
Arabidopsis thaliana
enzyme is involved in a polyamine back-conversion pathway
spermidine + 3-aminopropanal + H2O2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.5.3.16
recombinant protein
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.3.16
additional information
no activity with spermidine
676604
Arabidopsis thaliana
?
-
-
-
-
1.5.3.16
N1-acetylspermine + O2 + H2O
-
676604
Arabidopsis thaliana
spermidine + 3-acetamidopropanal + H2O2
-
-
-
?
1.5.3.16
norspermine + O2 + H2O
-
676604
Arabidopsis thaliana
?
-
-
-
?
1.5.3.16
spermine + O2 + H2O
-
676604
Arabidopsis thaliana
spermidine + 3-aminopropanal + H2O2
-
-
-
?
1.5.3.16
spermine + O2 + H2O
enzyme is involved in a polyamine back-conversion pathway
676604
Arabidopsis thaliana
spermidine + 3-aminopropanal + H2O2
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.5.3.16
0.2
-
N1-acetylspermine
pH 8.0
Arabidopsis thaliana
1.5.3.16
2.7
-
spermine
pH 8.0
Arabidopsis thaliana
1.5.3.16
6.9
-
norspermine
pH 8.0
Arabidopsis thaliana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.5.3.16
8
-
substrate: spermine or norspermine
Arabidopsis thaliana
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.5.3.16
7
9
pH 7: about 60% of maximal activity, pH 9.0: about 60% of maximal activity, substrate: spermine; pH 7: about 80% of maximal activity, pH 9.0: about 60% of maximal activity, substrate: norspermine
Arabidopsis thaliana